CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans

Construction of a genome-wide transgenic UAS-cDNA/ORF library in Drosophila based on the binary GAL4/UAS system has been severely hampered by technical difficulties, although genome-wide cDNA or ORF resources of Drosophila, human, and mouse have been publicly available for more than a decade. Here,...

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Detalles Bibliográficos
Autores principales: Wei, Ping, Xue, Wen, Zhao, Yun, Ning, Guang, Wang, Jiwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961578/
https://www.ncbi.nlm.nih.gov/pubmed/31722958
http://dx.doi.org/10.1101/gr.250811.119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961578/
https://www.ncbi.nlm.nih.gov/pubmed/31722958
http://dx.doi.org/10.1101/gr.250811.119

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