Copolymerization of single-cell nucleic acids into balls of acrylamide gel

We show the use of 5′-Acrydite oligonucleotides to copolymerize single-cell DNA or RNA into balls of acrylamide gel (BAGs). Combining this step with split-and-pool techniques for creating barcodes yields a method with advantages in cost and scalability, depth of coverage, ease of operation, minimal...

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Detalles Bibliográficos
Autores principales: Li, Siran, Kendall, Jude, Park, Sarah, Wang, Zihua, Alexander, Joan, Moffitt, Andrea, Ranade, Nissim, Danyko, Cassidy, Gegenhuber, Bruno, Fischer, Stephan, Robinson, Brian D., Lepor, Herbert, Tollkuhn, Jessica, Gillis, Jesse, Brouzes, Eric, Krasnitz, Alex, Levy, Dan, Wigler, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961581/
https://www.ncbi.nlm.nih.gov/pubmed/31727682
http://dx.doi.org/10.1101/gr.253047.119

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