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Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation
Graphene consists of two-dimensional sp2-bonded carbon sheets, a single or a few layers thick, which has attracted considerable interest in recent years due to its good conductivity and biocompatibility. Three-dimensional graphene foam (3DG) has been demonstrated to be a robust scaffold for culturin...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961593/ https://www.ncbi.nlm.nih.gov/pubmed/31998703 http://dx.doi.org/10.3389/fbioe.2019.00436 |
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author | Fang, Qiaojun Zhang, Yuhua Chen, Xiangbo Li, He Cheng, Liya Zhu, Wenjuan Zhang, Zhong Tang, Mingliang Liu, Wei Wang, Hui Wang, Tian Shen, Tie Chai, Renjie |
author_facet | Fang, Qiaojun Zhang, Yuhua Chen, Xiangbo Li, He Cheng, Liya Zhu, Wenjuan Zhang, Zhong Tang, Mingliang Liu, Wei Wang, Hui Wang, Tian Shen, Tie Chai, Renjie |
author_sort | Fang, Qiaojun |
collection | PubMed |
description | Graphene consists of two-dimensional sp2-bonded carbon sheets, a single or a few layers thick, which has attracted considerable interest in recent years due to its good conductivity and biocompatibility. Three-dimensional graphene foam (3DG) has been demonstrated to be a robust scaffold for culturing neural stem cells (NSCs) in vitro that not only supports NSCs growth, but also maintains cells in a more active proliferative state than 2D graphene films and ordinary glass. In addition, 3DG can enhance NSCs differentiation into astrocytes and especially neurons. However, the underlying mechanisms behind 3DG's effects are still poorly understood. Metabolism is the fundamental characteristic of life and provides substances for building and powering the cell. Metabolic activity is tightly tied with the proliferation, differentiation, and self-renewal of stem cells. This study focused on the metabolic reconfiguration of stem cells induced by culturing on 3DG. This study established the correlation between metabolic reconfiguration metabolomics with NSCs cell proliferation rate on different scaffold. Several metabolic processes have been uncovered in association with the proliferation change of NSCs. Especially, culturing on 3DG triggered pathways that increased amino acid incorporation and enhanced glucose metabolism. These data suggested a potential association between graphene and pathways involved in Parkinson's disease. Our work provides a very useful starting point for further studies of NSC fate determination on 3DG. |
format | Online Article Text |
id | pubmed-6961593 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69615932020-01-29 Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation Fang, Qiaojun Zhang, Yuhua Chen, Xiangbo Li, He Cheng, Liya Zhu, Wenjuan Zhang, Zhong Tang, Mingliang Liu, Wei Wang, Hui Wang, Tian Shen, Tie Chai, Renjie Front Bioeng Biotechnol Bioengineering and Biotechnology Graphene consists of two-dimensional sp2-bonded carbon sheets, a single or a few layers thick, which has attracted considerable interest in recent years due to its good conductivity and biocompatibility. Three-dimensional graphene foam (3DG) has been demonstrated to be a robust scaffold for culturing neural stem cells (NSCs) in vitro that not only supports NSCs growth, but also maintains cells in a more active proliferative state than 2D graphene films and ordinary glass. In addition, 3DG can enhance NSCs differentiation into astrocytes and especially neurons. However, the underlying mechanisms behind 3DG's effects are still poorly understood. Metabolism is the fundamental characteristic of life and provides substances for building and powering the cell. Metabolic activity is tightly tied with the proliferation, differentiation, and self-renewal of stem cells. This study focused on the metabolic reconfiguration of stem cells induced by culturing on 3DG. This study established the correlation between metabolic reconfiguration metabolomics with NSCs cell proliferation rate on different scaffold. Several metabolic processes have been uncovered in association with the proliferation change of NSCs. Especially, culturing on 3DG triggered pathways that increased amino acid incorporation and enhanced glucose metabolism. These data suggested a potential association between graphene and pathways involved in Parkinson's disease. Our work provides a very useful starting point for further studies of NSC fate determination on 3DG. Frontiers Media S.A. 2020-01-08 /pmc/articles/PMC6961593/ /pubmed/31998703 http://dx.doi.org/10.3389/fbioe.2019.00436 Text en Copyright © 2020 Fang, Zhang, Chen, Li, Cheng, Zhu, Zhang, Tang, Liu, Wang, Wang, Shen and Chai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Fang, Qiaojun Zhang, Yuhua Chen, Xiangbo Li, He Cheng, Liya Zhu, Wenjuan Zhang, Zhong Tang, Mingliang Liu, Wei Wang, Hui Wang, Tian Shen, Tie Chai, Renjie Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title | Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title_full | Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title_fullStr | Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title_full_unstemmed | Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title_short | Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation |
title_sort | three-dimensional graphene enhances neural stem cell proliferation through metabolic regulation |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961593/ https://www.ncbi.nlm.nih.gov/pubmed/31998703 http://dx.doi.org/10.3389/fbioe.2019.00436 |
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