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Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome
Vascular dysfunction predisposes to cardiovascular complications of metabolic syndrome (MetS). The current study investigated the mechanism(s) of curcumin’s (CUR) protective effect against vascular reactivity irregularities in MetS. MetS was induced by feeding rats on high fructose high salt diet. T...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962217/ https://www.ncbi.nlm.nih.gov/pubmed/31941978 http://dx.doi.org/10.1038/s41598-019-57268-z |
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author | Ahmed, Osama A. A. El-Bassossy, Hany M. Azhar, Ahmad S. Tarkhan, Mayada M. El-Mas, Mahmoud M. |
author_facet | Ahmed, Osama A. A. El-Bassossy, Hany M. Azhar, Ahmad S. Tarkhan, Mayada M. El-Mas, Mahmoud M. |
author_sort | Ahmed, Osama A. A. |
collection | PubMed |
description | Vascular dysfunction predisposes to cardiovascular complications of metabolic syndrome (MetS). The current study investigated the mechanism(s) of curcumin’s (CUR) protective effect against vascular reactivity irregularities in MetS. MetS was induced by feeding rats on high fructose high salt diet. Tension studies were undertaken in aortic rings to assess the influence of CUR on vasoconstrictor or vasorelaxant responses. The effect on advanced glycation endproducts (AGEs) was studied by incubating aortic tissues with methylglyoxal, the AGEs precursor, in the absence and presence of CUR. In addition, CUR effects on in-vitro generation of AGEs and diphenyl-2-picrylhydrazyl (DPPH) free radicals were studied. The incubation with CUR for 1 hr produced significant and concentration-dependent alleviation of the exaggerated vasoconstriction observed in aortas isolated from MetS, however failed to improve the concomitant attenuation of vasodilatory responses to ACh in PE-precontracted aortas. By contrast, CUR caused direct concentration-dependent vasodilations of precontracted aortas, effects that were blunted after nitric oxide synthase inhibition by L-NAME. Similar to its effects in MetS aortas, CUR alleviated exaggerated PE vasoconstriction but did not affect impaired ACh vasodilations in AGEs-exposed aortas. In addition, CUR showed significant dose-dependent DPPH free radicals scavenging activity and inhibited both MG and fructose induced AGEs formation at the level of protein oxidation step as evident from the effect on dityrosine and N-formylkyramine. CUR alleviates exaggerated vasoconstriction in MetS through interfering with AGEs formation and AGEs-induced vascular injury. Free radical scavenging and direct vasodilatory activities could also participate in the advantageous vascular actions of CUR. |
format | Online Article Text |
id | pubmed-6962217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69622172020-01-23 Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome Ahmed, Osama A. A. El-Bassossy, Hany M. Azhar, Ahmad S. Tarkhan, Mayada M. El-Mas, Mahmoud M. Sci Rep Article Vascular dysfunction predisposes to cardiovascular complications of metabolic syndrome (MetS). The current study investigated the mechanism(s) of curcumin’s (CUR) protective effect against vascular reactivity irregularities in MetS. MetS was induced by feeding rats on high fructose high salt diet. Tension studies were undertaken in aortic rings to assess the influence of CUR on vasoconstrictor or vasorelaxant responses. The effect on advanced glycation endproducts (AGEs) was studied by incubating aortic tissues with methylglyoxal, the AGEs precursor, in the absence and presence of CUR. In addition, CUR effects on in-vitro generation of AGEs and diphenyl-2-picrylhydrazyl (DPPH) free radicals were studied. The incubation with CUR for 1 hr produced significant and concentration-dependent alleviation of the exaggerated vasoconstriction observed in aortas isolated from MetS, however failed to improve the concomitant attenuation of vasodilatory responses to ACh in PE-precontracted aortas. By contrast, CUR caused direct concentration-dependent vasodilations of precontracted aortas, effects that were blunted after nitric oxide synthase inhibition by L-NAME. Similar to its effects in MetS aortas, CUR alleviated exaggerated PE vasoconstriction but did not affect impaired ACh vasodilations in AGEs-exposed aortas. In addition, CUR showed significant dose-dependent DPPH free radicals scavenging activity and inhibited both MG and fructose induced AGEs formation at the level of protein oxidation step as evident from the effect on dityrosine and N-formylkyramine. CUR alleviates exaggerated vasoconstriction in MetS through interfering with AGEs formation and AGEs-induced vascular injury. Free radical scavenging and direct vasodilatory activities could also participate in the advantageous vascular actions of CUR. Nature Publishing Group UK 2020-01-15 /pmc/articles/PMC6962217/ /pubmed/31941978 http://dx.doi.org/10.1038/s41598-019-57268-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ahmed, Osama A. A. El-Bassossy, Hany M. Azhar, Ahmad S. Tarkhan, Mayada M. El-Mas, Mahmoud M. Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title | Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title_full | Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title_fullStr | Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title_full_unstemmed | Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title_short | Interference with AGEs formation and AGEs-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
title_sort | interference with ages formation and ages-induced vascular injury mediates curcumin vascular protection in metabolic syndrome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962217/ https://www.ncbi.nlm.nih.gov/pubmed/31941978 http://dx.doi.org/10.1038/s41598-019-57268-z |
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