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Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus

MicroRNA-27a/b are small non-coding RNAs which are reported to regulate inflammatory response and cell proliferation. Although some studies have demonstrated that miR-27b is down-regulated in the oral specimens of patients suffering with oral lichen planus (OLP), the molecular mechanism of miR-27b d...

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Autores principales: Ge, Xuejun, Yuan, Lu, Wei, Jizhen, Nguyen, Tivoli, Tang, Chenwei, Liao, Wang, Li, Ran, Yang, Fang, Zhang, Fang, Zhao, Bin, Du, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962379/
https://www.ncbi.nlm.nih.gov/pubmed/31942011
http://dx.doi.org/10.1038/s41598-019-57288-9
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author Ge, Xuejun
Yuan, Lu
Wei, Jizhen
Nguyen, Tivoli
Tang, Chenwei
Liao, Wang
Li, Ran
Yang, Fang
Zhang, Fang
Zhao, Bin
Du, Jie
author_facet Ge, Xuejun
Yuan, Lu
Wei, Jizhen
Nguyen, Tivoli
Tang, Chenwei
Liao, Wang
Li, Ran
Yang, Fang
Zhang, Fang
Zhao, Bin
Du, Jie
author_sort Ge, Xuejun
collection PubMed
description MicroRNA-27a/b are small non-coding RNAs which are reported to regulate inflammatory response and cell proliferation. Although some studies have demonstrated that miR-27b is down-regulated in the oral specimens of patients suffering with oral lichen planus (OLP), the molecular mechanism of miR-27b decrease remains a large mystery, and the expression of miR-27a in OLP is not well explored. Here, we demonstrated both miR-27a and miR-27b, compared with healthy controls, were reduced in the oral biopsies, serum and saliva samples derived from OLP patients. The reductions of miR-27a/b were also confirmed in the lipopolysaccharide (LPS)- or activated CD4(+) T cell-treated human oral keratinocytes (HOKs). Furthermore, we found vitamin D receptor (VDR) binding sites in the promoters of miR-27a/b genes and verified this finding. We also tested miR-27a/b levels in the oral epithelium from paricalcitol-treated, vitamin D deficient or VDR knockout mice. In the rescue experiments, we confirmed vitamin D and VDR inhibited LPS- or activated CD4(+) T cell-induced miR-27a/b reductions in HOKs. In sum, our results show that vitamin D/VDR signaling induces miR-27a/b in oral lichen planus.
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spelling pubmed-69623792020-01-23 Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus Ge, Xuejun Yuan, Lu Wei, Jizhen Nguyen, Tivoli Tang, Chenwei Liao, Wang Li, Ran Yang, Fang Zhang, Fang Zhao, Bin Du, Jie Sci Rep Article MicroRNA-27a/b are small non-coding RNAs which are reported to regulate inflammatory response and cell proliferation. Although some studies have demonstrated that miR-27b is down-regulated in the oral specimens of patients suffering with oral lichen planus (OLP), the molecular mechanism of miR-27b decrease remains a large mystery, and the expression of miR-27a in OLP is not well explored. Here, we demonstrated both miR-27a and miR-27b, compared with healthy controls, were reduced in the oral biopsies, serum and saliva samples derived from OLP patients. The reductions of miR-27a/b were also confirmed in the lipopolysaccharide (LPS)- or activated CD4(+) T cell-treated human oral keratinocytes (HOKs). Furthermore, we found vitamin D receptor (VDR) binding sites in the promoters of miR-27a/b genes and verified this finding. We also tested miR-27a/b levels in the oral epithelium from paricalcitol-treated, vitamin D deficient or VDR knockout mice. In the rescue experiments, we confirmed vitamin D and VDR inhibited LPS- or activated CD4(+) T cell-induced miR-27a/b reductions in HOKs. In sum, our results show that vitamin D/VDR signaling induces miR-27a/b in oral lichen planus. Nature Publishing Group UK 2020-01-15 /pmc/articles/PMC6962379/ /pubmed/31942011 http://dx.doi.org/10.1038/s41598-019-57288-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ge, Xuejun
Yuan, Lu
Wei, Jizhen
Nguyen, Tivoli
Tang, Chenwei
Liao, Wang
Li, Ran
Yang, Fang
Zhang, Fang
Zhao, Bin
Du, Jie
Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title_full Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title_fullStr Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title_full_unstemmed Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title_short Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
title_sort vitamin d/vdr signaling induces mir-27a/b expression in oral lichen planus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962379/
https://www.ncbi.nlm.nih.gov/pubmed/31942011
http://dx.doi.org/10.1038/s41598-019-57288-9
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