Cargando…

Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture

Tuberculosis (TB) is the leading cause of death globally, surpassing HIV. Furthermore, multidrug-resistant and extensively drug-resistant TB have become global public health threats. Care of TB patients starts with quality, accessible, and affordable diagnosis. The study presents a novel technique c...

Descripción completa

Detalles Bibliográficos
Autores principales: Briceno, Ruben Kenny, Sergent, Shane Ryan, Benites, Santiago Moises, Alocilja, Evangelyn C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963232/
https://www.ncbi.nlm.nih.gov/pubmed/31847171
http://dx.doi.org/10.3390/diagnostics9040222
_version_ 1783488239172059136
author Briceno, Ruben Kenny
Sergent, Shane Ryan
Benites, Santiago Moises
Alocilja, Evangelyn C.
author_facet Briceno, Ruben Kenny
Sergent, Shane Ryan
Benites, Santiago Moises
Alocilja, Evangelyn C.
author_sort Briceno, Ruben Kenny
collection PubMed
description Tuberculosis (TB) is the leading cause of death globally, surpassing HIV. Furthermore, multidrug-resistant and extensively drug-resistant TB have become global public health threats. Care of TB patients starts with quality, accessible, and affordable diagnosis. The study presents a novel technique called nanoparticle-based colorimetric biosensing assay (NCBA) based on the principles of magnetically activated cell enrichment. A total of 1108 sputum samples were subjected to sputum smear microscopy (SSM), NCBA, and standard culture. SSM and NCBA were completed in 20 min; culture was completed in 8 weeks. Results show that NCBA has matching sensitivity of 100.0% and specificity of 99.7% compared to the gold standard culture method at a cost of $0.50/test based on Peruvian conditions. Sputum smear microscopy has 63.87% sensitivity compared to culture. NCBA has the potential of being used in local health clinics as it only requires a microscope that is widely available in many rural areas. Because NCBA could detect low levels of bacterial load comparable to culture, it could be used for rapid and early TB-onset detection. The gain in time is critical as TB is airborne and highly infectious, minimizing contact exposure. Early detection could lead to early treatment, while the patient’s immune system is still high. The low cost makes NCBA affordable and accessible to those who need them the most.
format Online
Article
Text
id pubmed-6963232
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69632322020-01-27 Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture Briceno, Ruben Kenny Sergent, Shane Ryan Benites, Santiago Moises Alocilja, Evangelyn C. Diagnostics (Basel) Article Tuberculosis (TB) is the leading cause of death globally, surpassing HIV. Furthermore, multidrug-resistant and extensively drug-resistant TB have become global public health threats. Care of TB patients starts with quality, accessible, and affordable diagnosis. The study presents a novel technique called nanoparticle-based colorimetric biosensing assay (NCBA) based on the principles of magnetically activated cell enrichment. A total of 1108 sputum samples were subjected to sputum smear microscopy (SSM), NCBA, and standard culture. SSM and NCBA were completed in 20 min; culture was completed in 8 weeks. Results show that NCBA has matching sensitivity of 100.0% and specificity of 99.7% compared to the gold standard culture method at a cost of $0.50/test based on Peruvian conditions. Sputum smear microscopy has 63.87% sensitivity compared to culture. NCBA has the potential of being used in local health clinics as it only requires a microscope that is widely available in many rural areas. Because NCBA could detect low levels of bacterial load comparable to culture, it could be used for rapid and early TB-onset detection. The gain in time is critical as TB is airborne and highly infectious, minimizing contact exposure. Early detection could lead to early treatment, while the patient’s immune system is still high. The low cost makes NCBA affordable and accessible to those who need them the most. MDPI 2019-12-13 /pmc/articles/PMC6963232/ /pubmed/31847171 http://dx.doi.org/10.3390/diagnostics9040222 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Briceno, Ruben Kenny
Sergent, Shane Ryan
Benites, Santiago Moises
Alocilja, Evangelyn C.
Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title_full Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title_fullStr Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title_full_unstemmed Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title_short Nanoparticle-Based Biosensing Assay for Universally Accessible Low-Cost TB Detection with Comparable Sensitivity as Culture
title_sort nanoparticle-based biosensing assay for universally accessible low-cost tb detection with comparable sensitivity as culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963232/
https://www.ncbi.nlm.nih.gov/pubmed/31847171
http://dx.doi.org/10.3390/diagnostics9040222
work_keys_str_mv AT bricenorubenkenny nanoparticlebasedbiosensingassayforuniversallyaccessiblelowcosttbdetectionwithcomparablesensitivityasculture
AT sergentshaneryan nanoparticlebasedbiosensingassayforuniversallyaccessiblelowcosttbdetectionwithcomparablesensitivityasculture
AT benitessantiagomoises nanoparticlebasedbiosensingassayforuniversallyaccessiblelowcosttbdetectionwithcomparablesensitivityasculture
AT alociljaevangelync nanoparticlebasedbiosensingassayforuniversallyaccessiblelowcosttbdetectionwithcomparablesensitivityasculture