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AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana
Because they are sessile organisms, plants need rapid and finely tuned signaling pathways to adapt to adverse environments, including salt stress. In this study, we identified a gene named Arabidopsis thaliana stress-induced BTB protein 1 (AtSIBP1), which encodes a nucleus protein with a BTB domain...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963258/ https://www.ncbi.nlm.nih.gov/pubmed/31817461 http://dx.doi.org/10.3390/plants8120573 |
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author | Wan, Xia Peng, Lu Xiong, Jie Li, Xiaoyi Wang, Jianmei Li, Xufeng Yang, Yi |
author_facet | Wan, Xia Peng, Lu Xiong, Jie Li, Xiaoyi Wang, Jianmei Li, Xufeng Yang, Yi |
author_sort | Wan, Xia |
collection | PubMed |
description | Because they are sessile organisms, plants need rapid and finely tuned signaling pathways to adapt to adverse environments, including salt stress. In this study, we identified a gene named Arabidopsis thaliana stress-induced BTB protein 1 (AtSIBP1), which encodes a nucleus protein with a BTB domain in its C-terminal side and is induced by salt and other stresses. The expression of the β-glucuronidase (GUS) gene driven by the AtSIBP1 promoter was found to be significantly induced in the presence of NaCl. The sibp1 mutant that lost AtSIBP1 function was found to be highly sensitive to salt stress and more vulnerable to salt stress than the wild type WT, while the overexpression of AtSIBP1 transgenic plants exhibited more tolerance to salt stress. According to the DAB staining, the sibp1 mutant accumulated more reactive oxygen species (ROS) than the WT and AtSIBP1 overexpression plants after salt stress. In addition, the expression levels of stress-induced marker genes in AtSIBP1 overexpression plants were markedly higher than those in the WT and sibp1 mutant plants. Therefore, our results demonstrate that AtSIBP1 was a positive regulator in salinity responses in Arabidopsis. |
format | Online Article Text |
id | pubmed-6963258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69632582020-01-27 AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana Wan, Xia Peng, Lu Xiong, Jie Li, Xiaoyi Wang, Jianmei Li, Xufeng Yang, Yi Plants (Basel) Article Because they are sessile organisms, plants need rapid and finely tuned signaling pathways to adapt to adverse environments, including salt stress. In this study, we identified a gene named Arabidopsis thaliana stress-induced BTB protein 1 (AtSIBP1), which encodes a nucleus protein with a BTB domain in its C-terminal side and is induced by salt and other stresses. The expression of the β-glucuronidase (GUS) gene driven by the AtSIBP1 promoter was found to be significantly induced in the presence of NaCl. The sibp1 mutant that lost AtSIBP1 function was found to be highly sensitive to salt stress and more vulnerable to salt stress than the wild type WT, while the overexpression of AtSIBP1 transgenic plants exhibited more tolerance to salt stress. According to the DAB staining, the sibp1 mutant accumulated more reactive oxygen species (ROS) than the WT and AtSIBP1 overexpression plants after salt stress. In addition, the expression levels of stress-induced marker genes in AtSIBP1 overexpression plants were markedly higher than those in the WT and sibp1 mutant plants. Therefore, our results demonstrate that AtSIBP1 was a positive regulator in salinity responses in Arabidopsis. MDPI 2019-12-05 /pmc/articles/PMC6963258/ /pubmed/31817461 http://dx.doi.org/10.3390/plants8120573 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wan, Xia Peng, Lu Xiong, Jie Li, Xiaoyi Wang, Jianmei Li, Xufeng Yang, Yi AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title | AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title_full | AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title_fullStr | AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title_full_unstemmed | AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title_short | AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana |
title_sort | atsibp1, a novel btb domain-containing protein, positively regulates salt signaling in arabidopsis thaliana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963258/ https://www.ncbi.nlm.nih.gov/pubmed/31817461 http://dx.doi.org/10.3390/plants8120573 |
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