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Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules

Peels from the inner epidermis of onion bulbs are a model system in plant cell biology. While the inner epidermis of red onions is characteristically white, small patches of cells sometimes redden, containing vacuolar anthocyanin. This study investigated the spectroscopic properties of these anthocy...

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Autor principal: Collings, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963288/
https://www.ncbi.nlm.nih.gov/pubmed/31842412
http://dx.doi.org/10.3390/plants8120596
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author Collings, David A.
author_facet Collings, David A.
author_sort Collings, David A.
collection PubMed
description Peels from the inner epidermis of onion bulbs are a model system in plant cell biology. While the inner epidermis of red onions is characteristically white, small patches of cells sometimes redden, containing vacuolar anthocyanin. This study investigated the spectroscopic properties of these anthocyanic cells. When fluorescent dyes were loaded into the vacuole of onion epidermal cells, the anthocyanic cells showed decreased dye fluorescence. This decrease was observed for fluorescein and carboxyfluorescein that are pumped into the vacuole by anion transporters, for acridine orange which acid loads into the vacuole, and for the fluorescent sugar analogue esculin loaded into the vacuole by sucrose transporters. Similar decreases in carboxyfluorescein fluorescence were observed when dye was loaded into the vacuoles of several other plant species, but decreases were not observed for dyes resident in the tonoplast membrane. As cellular physiology was unaffected in the anthocyanic cells, with cytoplasmic streaming, vacuolar and cytoplasmic pH not being altered, the decreased dye fluorescence from the anthocyanic cells can be attributed to fluorescence quenching. Furthermore, because quenching decreased with increasing temperature. It was concluded, therefore, that vacuolar anthocyanin can statically quench other fluorescent molecules in vivo, an effect previously demonstrated for anthocyanin in vitro.
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spelling pubmed-69632882020-02-26 Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules Collings, David A. Plants (Basel) Article Peels from the inner epidermis of onion bulbs are a model system in plant cell biology. While the inner epidermis of red onions is characteristically white, small patches of cells sometimes redden, containing vacuolar anthocyanin. This study investigated the spectroscopic properties of these anthocyanic cells. When fluorescent dyes were loaded into the vacuole of onion epidermal cells, the anthocyanic cells showed decreased dye fluorescence. This decrease was observed for fluorescein and carboxyfluorescein that are pumped into the vacuole by anion transporters, for acridine orange which acid loads into the vacuole, and for the fluorescent sugar analogue esculin loaded into the vacuole by sucrose transporters. Similar decreases in carboxyfluorescein fluorescence were observed when dye was loaded into the vacuoles of several other plant species, but decreases were not observed for dyes resident in the tonoplast membrane. As cellular physiology was unaffected in the anthocyanic cells, with cytoplasmic streaming, vacuolar and cytoplasmic pH not being altered, the decreased dye fluorescence from the anthocyanic cells can be attributed to fluorescence quenching. Furthermore, because quenching decreased with increasing temperature. It was concluded, therefore, that vacuolar anthocyanin can statically quench other fluorescent molecules in vivo, an effect previously demonstrated for anthocyanin in vitro. MDPI 2019-12-12 /pmc/articles/PMC6963288/ /pubmed/31842412 http://dx.doi.org/10.3390/plants8120596 Text en © 2019 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Collings, David A.
Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title_full Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title_fullStr Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title_full_unstemmed Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title_short Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules
title_sort anthocyanin in the vacuole of red onion epidermal cells quenches other fluorescent molecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963288/
https://www.ncbi.nlm.nih.gov/pubmed/31842412
http://dx.doi.org/10.3390/plants8120596
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