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The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt
The emergence of bla(KPC-2) and bla(NDM-1) producing Klebsiella pneumoniae represents a great problem in many Egyptian hospitals. One hundred and twenty-six K. pneumoniae isolates from patients admitted to Assiut University Hospital were identified by an API20E kit. Carbapenemase-producing K. pneumo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963397/ https://www.ncbi.nlm.nih.gov/pubmed/31847288 http://dx.doi.org/10.3390/antibiotics8040266 |
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author | Ramadan Mohamed, Eman Ali, Mamdouh Yones Waly, Nancy G F M Halby, Hamada Mohamed Abd El-Baky, Rehab Mahmoud |
author_facet | Ramadan Mohamed, Eman Ali, Mamdouh Yones Waly, Nancy G F M Halby, Hamada Mohamed Abd El-Baky, Rehab Mahmoud |
author_sort | Ramadan Mohamed, Eman |
collection | PubMed |
description | The emergence of bla(KPC-2) and bla(NDM-1) producing Klebsiella pneumoniae represents a great problem in many Egyptian hospitals. One hundred and twenty-six K. pneumoniae isolates from patients admitted to Assiut University Hospital were identified by an API20E kit. Carbapenemase-producing K. pneumoniae (CPKP) was detected by the modified carbapenem inactivation method (mCIM), the EDTA-modified carbapenem inactivation method (eCIM), and an E-test. Based on the polymerase chain reaction, all isolates were negative for bla-(VIM-1) and bla-(IMP-1), fifteen of these isolates were positive for both bla(KPC-2) and bla(NDM-1), two isolates were positive for bla(KPC-2) only, and twenty-eight isolates were positive for bla-(NDM-1) only. Although one isolate was positive for the string test, all CPKP isolates were negative for capsular genes. Only 71.1% of CPKP transferred their plasmids to their corresponding transconjugants (E. coli J53). The resistance patterns of the clinical isolates and their transconjugates were similar, except for 12 isolates, which showed differences with their transconjugates in the resistance profile of four antibiotics. Molecular typing of the plasmids based on replicon typing showed that Inc FIIK and FII plasmids predominated in isolates and their transconjugants carrying bla(KPC-2) and/or bla(NDM-1). Conjugative Inc FII plasmids play an important role in the spread of CPKP, and their recognition is essential to limit their spread. |
format | Online Article Text |
id | pubmed-6963397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69633972020-02-26 The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt Ramadan Mohamed, Eman Ali, Mamdouh Yones Waly, Nancy G F M Halby, Hamada Mohamed Abd El-Baky, Rehab Mahmoud Antibiotics (Basel) Article The emergence of bla(KPC-2) and bla(NDM-1) producing Klebsiella pneumoniae represents a great problem in many Egyptian hospitals. One hundred and twenty-six K. pneumoniae isolates from patients admitted to Assiut University Hospital were identified by an API20E kit. Carbapenemase-producing K. pneumoniae (CPKP) was detected by the modified carbapenem inactivation method (mCIM), the EDTA-modified carbapenem inactivation method (eCIM), and an E-test. Based on the polymerase chain reaction, all isolates were negative for bla-(VIM-1) and bla-(IMP-1), fifteen of these isolates were positive for both bla(KPC-2) and bla(NDM-1), two isolates were positive for bla(KPC-2) only, and twenty-eight isolates were positive for bla-(NDM-1) only. Although one isolate was positive for the string test, all CPKP isolates were negative for capsular genes. Only 71.1% of CPKP transferred their plasmids to their corresponding transconjugants (E. coli J53). The resistance patterns of the clinical isolates and their transconjugates were similar, except for 12 isolates, which showed differences with their transconjugates in the resistance profile of four antibiotics. Molecular typing of the plasmids based on replicon typing showed that Inc FIIK and FII plasmids predominated in isolates and their transconjugants carrying bla(KPC-2) and/or bla(NDM-1). Conjugative Inc FII plasmids play an important role in the spread of CPKP, and their recognition is essential to limit their spread. MDPI 2019-12-13 /pmc/articles/PMC6963397/ /pubmed/31847288 http://dx.doi.org/10.3390/antibiotics8040266 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ramadan Mohamed, Eman Ali, Mamdouh Yones Waly, Nancy G F M Halby, Hamada Mohamed Abd El-Baky, Rehab Mahmoud The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title | The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title_full | The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title_fullStr | The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title_full_unstemmed | The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title_short | The Inc FII Plasmid and its Contribution in the Transmission of bla(NDM-1) and bla(KPC-2) in Klebsiella pneumoniae in Egypt |
title_sort | inc fii plasmid and its contribution in the transmission of bla(ndm-1) and bla(kpc-2) in klebsiella pneumoniae in egypt |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963397/ https://www.ncbi.nlm.nih.gov/pubmed/31847288 http://dx.doi.org/10.3390/antibiotics8040266 |
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