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Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress
Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963410/ https://www.ncbi.nlm.nih.gov/pubmed/31888189 http://dx.doi.org/10.3390/plants8120610 |
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author | Varghese, Nisha Alyammahi, Onoud Nasreddine, Sarah Alhassani, Abla Gururani, Mayank Anand |
author_facet | Varghese, Nisha Alyammahi, Onoud Nasreddine, Sarah Alhassani, Abla Gururani, Mayank Anand |
author_sort | Varghese, Nisha |
collection | PubMed |
description | Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity stress in Avena sativa (oat) plants. Oat plants were exposed to four treatments (three replicate pots per treatment): well-watered (WW; control); watered with 300 mM salt solution for 10 days (NaCl); pretreated with 100 µM melatonin solution for 7 days then watered normally for 10 days (Mel+W); or pretreated with 100 µM melatonin for 7 days then 300 mM salt for 10 days (Mel+NaCl). Considerable differences in growth parameters, chlorophyll content, stomatal conductance, proline accumulation, lipid peroxidation, electrolyte leakage, and growth parameters were observed between groups. Genes encoding three major antioxidant enzymes were upregulated in the Mel+NaCl group compared to the other groups. Chlorophyll-a fluorescence kinetic analyses revealed that almost all photosynthetic parameters were improved in Mel+NaCl plants compared to the other treatments. Analysis of genes encoding the major extrinsic proteins of photosystem II (PSII) revealed that PsbA, PsbB, PsbC, and PsbD (but not PsbO) were highly upregulated in Mel+NaCl plants compared to the other groups, indicating melatonin positively influenced photosynthesis under control conditions and salt stress. In addition, melatonin upregulated stress-responsive NAC transcription factor genes in plants exposed to salt stress. These findings suggest melatonin pretreatment improves photosynthesis and enhances salt tolerance in oat plants. |
format | Online Article Text |
id | pubmed-6963410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69634102020-02-26 Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress Varghese, Nisha Alyammahi, Onoud Nasreddine, Sarah Alhassani, Abla Gururani, Mayank Anand Plants (Basel) Article Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity stress in Avena sativa (oat) plants. Oat plants were exposed to four treatments (three replicate pots per treatment): well-watered (WW; control); watered with 300 mM salt solution for 10 days (NaCl); pretreated with 100 µM melatonin solution for 7 days then watered normally for 10 days (Mel+W); or pretreated with 100 µM melatonin for 7 days then 300 mM salt for 10 days (Mel+NaCl). Considerable differences in growth parameters, chlorophyll content, stomatal conductance, proline accumulation, lipid peroxidation, electrolyte leakage, and growth parameters were observed between groups. Genes encoding three major antioxidant enzymes were upregulated in the Mel+NaCl group compared to the other groups. Chlorophyll-a fluorescence kinetic analyses revealed that almost all photosynthetic parameters were improved in Mel+NaCl plants compared to the other treatments. Analysis of genes encoding the major extrinsic proteins of photosystem II (PSII) revealed that PsbA, PsbB, PsbC, and PsbD (but not PsbO) were highly upregulated in Mel+NaCl plants compared to the other groups, indicating melatonin positively influenced photosynthesis under control conditions and salt stress. In addition, melatonin upregulated stress-responsive NAC transcription factor genes in plants exposed to salt stress. These findings suggest melatonin pretreatment improves photosynthesis and enhances salt tolerance in oat plants. MDPI 2019-12-16 /pmc/articles/PMC6963410/ /pubmed/31888189 http://dx.doi.org/10.3390/plants8120610 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Varghese, Nisha Alyammahi, Onoud Nasreddine, Sarah Alhassani, Abla Gururani, Mayank Anand Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title | Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title_full | Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title_fullStr | Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title_full_unstemmed | Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title_short | Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress |
title_sort | melatonin positively influences the photosynthetic machinery and antioxidant system of avena sativa during salinity stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963410/ https://www.ncbi.nlm.nih.gov/pubmed/31888189 http://dx.doi.org/10.3390/plants8120610 |
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