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Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes

The objective of the study was the investigation of the behaviour of cathelicidin-1 in the milk after experimental infection with two prominent bacterial pathogens (experiment 1: Mannheimia haemolytica, experiment 2: M. haemolytica and Staphylococcus chromogenes) as a potential early indicator for d...

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Autores principales: Katsafadou, Angeliki I., Tsangaris, George Th., Vasileiou, Natalia G.C., Ioannidi, Katerina S., Anagnostopoulos, Athanasios K., Billinis, Charalambos, Fragkou, Ilektra A., Papadopoulos, Elias, Mavrogianni, Vasia S., Michael, Charalambia K., Addis, M. Filippa, Fthenakis, George C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963440/
https://www.ncbi.nlm.nih.gov/pubmed/31795190
http://dx.doi.org/10.3390/pathogens8040270
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author Katsafadou, Angeliki I.
Tsangaris, George Th.
Vasileiou, Natalia G.C.
Ioannidi, Katerina S.
Anagnostopoulos, Athanasios K.
Billinis, Charalambos
Fragkou, Ilektra A.
Papadopoulos, Elias
Mavrogianni, Vasia S.
Michael, Charalambia K.
Addis, M. Filippa
Fthenakis, George C.
author_facet Katsafadou, Angeliki I.
Tsangaris, George Th.
Vasileiou, Natalia G.C.
Ioannidi, Katerina S.
Anagnostopoulos, Athanasios K.
Billinis, Charalambos
Fragkou, Ilektra A.
Papadopoulos, Elias
Mavrogianni, Vasia S.
Michael, Charalambia K.
Addis, M. Filippa
Fthenakis, George C.
author_sort Katsafadou, Angeliki I.
collection PubMed
description The objective of the study was the investigation of the behaviour of cathelicidin-1 in the milk after experimental infection with two prominent bacterial pathogens (experiment 1: Mannheimia haemolytica, experiment 2: M. haemolytica and Staphylococcus chromogenes) as a potential early indicator for diagnosis of mastitis in sheep. In two experiments, after bacterial inoculation into the udder of ewes, bacteriological and cytological examinations of milk samples as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially. Cathelicidin-1 was detected and spot densities obtained from PDQuest v.8.0 were recorded. Associations were calculated between cell content and spot densities as well as between presence of mastitis in a mammary gland at a given time-point and detection of cathelicidin-1 in the respective milk sample. All inoculated mammary glands developed mastitis, confirmed by the consistent bacterial isolation from mammary secretion and increased leucocyte content therein. Spot density of cathelicidin-1 in samples from inoculated glands increased 3 h post-inoculation; spot density of cathelicidin-1 in samples from inoculated glands was higher than in samples from uninoculated controls. There was clear evidence of correlation between cell content and cathelicidin-1 spot densities in milk samples. There was significant association between presence of mastitis in the mammary gland and detection of cathelicidin-1 in the respective milk sample; overall accuracy was 0.818—this was significantly greater during the first 24 h post-challenge (0.903) than after the first day (0.704). In conclusion, detection of cathelicidin-1 in milk was significantly associated with presence of mastitis in ewes. The associations were stronger during the first 24 h post-infection than after the first day. Cathelicidin-1 has the advantage that it can be a non-specific biomarker, as simply a “positive”/“negative” assessment would be sufficient.
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spelling pubmed-69634402020-02-26 Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes Katsafadou, Angeliki I. Tsangaris, George Th. Vasileiou, Natalia G.C. Ioannidi, Katerina S. Anagnostopoulos, Athanasios K. Billinis, Charalambos Fragkou, Ilektra A. Papadopoulos, Elias Mavrogianni, Vasia S. Michael, Charalambia K. Addis, M. Filippa Fthenakis, George C. Pathogens Article The objective of the study was the investigation of the behaviour of cathelicidin-1 in the milk after experimental infection with two prominent bacterial pathogens (experiment 1: Mannheimia haemolytica, experiment 2: M. haemolytica and Staphylococcus chromogenes) as a potential early indicator for diagnosis of mastitis in sheep. In two experiments, after bacterial inoculation into the udder of ewes, bacteriological and cytological examinations of milk samples as well as proteomics examinations [two-dimensional gel electrophoresis analysis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis] were performed sequentially. Cathelicidin-1 was detected and spot densities obtained from PDQuest v.8.0 were recorded. Associations were calculated between cell content and spot densities as well as between presence of mastitis in a mammary gland at a given time-point and detection of cathelicidin-1 in the respective milk sample. All inoculated mammary glands developed mastitis, confirmed by the consistent bacterial isolation from mammary secretion and increased leucocyte content therein. Spot density of cathelicidin-1 in samples from inoculated glands increased 3 h post-inoculation; spot density of cathelicidin-1 in samples from inoculated glands was higher than in samples from uninoculated controls. There was clear evidence of correlation between cell content and cathelicidin-1 spot densities in milk samples. There was significant association between presence of mastitis in the mammary gland and detection of cathelicidin-1 in the respective milk sample; overall accuracy was 0.818—this was significantly greater during the first 24 h post-challenge (0.903) than after the first day (0.704). In conclusion, detection of cathelicidin-1 in milk was significantly associated with presence of mastitis in ewes. The associations were stronger during the first 24 h post-infection than after the first day. Cathelicidin-1 has the advantage that it can be a non-specific biomarker, as simply a “positive”/“negative” assessment would be sufficient. MDPI 2019-11-28 /pmc/articles/PMC6963440/ /pubmed/31795190 http://dx.doi.org/10.3390/pathogens8040270 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Katsafadou, Angeliki I.
Tsangaris, George Th.
Vasileiou, Natalia G.C.
Ioannidi, Katerina S.
Anagnostopoulos, Athanasios K.
Billinis, Charalambos
Fragkou, Ilektra A.
Papadopoulos, Elias
Mavrogianni, Vasia S.
Michael, Charalambia K.
Addis, M. Filippa
Fthenakis, George C.
Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title_full Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title_fullStr Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title_full_unstemmed Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title_short Detection of Cathelicidin-1 in the Milk as an Early Indicator of Mastitis in Ewes
title_sort detection of cathelicidin-1 in the milk as an early indicator of mastitis in ewes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963440/
https://www.ncbi.nlm.nih.gov/pubmed/31795190
http://dx.doi.org/10.3390/pathogens8040270
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