Cargando…

Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease

Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis o...

Descripción completa

Detalles Bibliográficos
Autores principales: Johnson-Mackinnon, Jessica C., Crosbie, Philip B. B., Karlsbakk, Egil, Marcos-Lopez, Mar, Paley, Richard, Nowak, Barbara F., Bridle, Andrew R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/
https://www.ncbi.nlm.nih.gov/pubmed/31752364
http://dx.doi.org/10.3390/pathogens8040244
_version_ 1783488315400388608
author Johnson-Mackinnon, Jessica C.
Crosbie, Philip B. B.
Karlsbakk, Egil
Marcos-Lopez, Mar
Paley, Richard
Nowak, Barbara F.
Bridle, Andrew R.
author_facet Johnson-Mackinnon, Jessica C.
Crosbie, Philip B. B.
Karlsbakk, Egil
Marcos-Lopez, Mar
Paley, Richard
Nowak, Barbara F.
Bridle, Andrew R.
author_sort Johnson-Mackinnon, Jessica C.
collection PubMed
description Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area.
format Online
Article
Text
id pubmed-6963586
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69635862020-01-30 Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease Johnson-Mackinnon, Jessica C. Crosbie, Philip B. B. Karlsbakk, Egil Marcos-Lopez, Mar Paley, Richard Nowak, Barbara F. Bridle, Andrew R. Pathogens Article Neoparamoba perurans, is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to N. perurans with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of N. perurans from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area. MDPI 2019-11-19 /pmc/articles/PMC6963586/ /pubmed/31752364 http://dx.doi.org/10.3390/pathogens8040244 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Johnson-Mackinnon, Jessica C.
Crosbie, Philip B. B.
Karlsbakk, Egil
Marcos-Lopez, Mar
Paley, Richard
Nowak, Barbara F.
Bridle, Andrew R.
Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title_full Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title_fullStr Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title_full_unstemmed Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title_short Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates of Neoparamoeba perurans, the Causative Agent of Amoebic Gill Disease
title_sort multilocus sequence typing (mlst) and random polymorphic dna (rapd) comparisons of geographic isolates of neoparamoeba perurans, the causative agent of amoebic gill disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963586/
https://www.ncbi.nlm.nih.gov/pubmed/31752364
http://dx.doi.org/10.3390/pathogens8040244
work_keys_str_mv AT johnsonmackinnonjessicac multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT crosbiephilipbb multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT karlsbakkegil multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT marcoslopezmar multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT paleyrichard multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT nowakbarbaraf multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease
AT bridleandrewr multilocussequencetypingmlstandrandompolymorphicdnarapdcomparisonsofgeographicisolatesofneoparamoebaperuransthecausativeagentofamoebicgilldisease