Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian count...

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Detalles Bibliográficos
Autores principales: Piamsomboon, Patharapol, Choi, Seong-Kyoon, Hanggono, Bambang, Nuraini, Yani Lestari, Wati, Fatma, Tang, Kathy F. J., Park, Song, Kwak, Dongmi, Rhee, Man Hee, Han, Jee Eun, Kim, Ji Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963587/
https://www.ncbi.nlm.nih.gov/pubmed/31726681
http://dx.doi.org/10.3390/pathogens8040233
Descripción
Sumario:We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10(6)–10(8) copies mg(−1)), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10(1)–10(3) copies mg(−1) or mL(−1) of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

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