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Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever

Coxiella burnetii is the causative agent of acute and chronic Q fever in humans. Although the isolates studied so far showed a difference in virulence potential between those causing the two forms of the disease, implying a difference in their proteomic profile, the methods used so far to diagnose t...

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Autores principales: Vranakis, Iosif, Mathioudaki, Eirini, Kokkini, Sofia, Psaroulaki, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963606/
https://www.ncbi.nlm.nih.gov/pubmed/31752191
http://dx.doi.org/10.3390/pathogens8040242
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author Vranakis, Iosif
Mathioudaki, Eirini
Kokkini, Sofia
Psaroulaki, Anna
author_facet Vranakis, Iosif
Mathioudaki, Eirini
Kokkini, Sofia
Psaroulaki, Anna
author_sort Vranakis, Iosif
collection PubMed
description Coxiella burnetii is the causative agent of acute and chronic Q fever in humans. Although the isolates studied so far showed a difference in virulence potential between those causing the two forms of the disease, implying a difference in their proteomic profile, the methods used so far to diagnose the two forms of the disease do not provide sufficient discriminatory capability, and human infections may be often misdiagnosed. The aim of the current study was to identify the outer membrane Com1 (CBU_1910) as a candidate protein for serodiagnostics of Q fever. The protein was cloned, expressed, purified, and used as an antigen in ELISA. The protein was then used for the screening of sera from patients suffering from chronic Q fever endocarditis, patients whose samples were negative for phase I immunoglobulin G (IgG), patients for whom at least one sample was positive for phase I IgG, and patients suffering from any kind of rheumatoid disease. Blood donors were used as the control group. Following statistical analysis, 92.4% (122/132) of the samples tested agreed with the negative clinical diagnosis, and 72.2% (26/36) agreed with the positive clinical diagnosis. Moreover, a significant correlation to the presence of the disease (p = 0.00) was calculated. The results support the idea that a Com1 antigen-based serodiagnostic test may be useful for differential diagnosis of chronic Q fever. Further studies are required to compare more immunogenic proteins of the bacterium against samples originating from patients suffering from different forms of the disease.
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spelling pubmed-69636062020-01-27 Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever Vranakis, Iosif Mathioudaki, Eirini Kokkini, Sofia Psaroulaki, Anna Pathogens Article Coxiella burnetii is the causative agent of acute and chronic Q fever in humans. Although the isolates studied so far showed a difference in virulence potential between those causing the two forms of the disease, implying a difference in their proteomic profile, the methods used so far to diagnose the two forms of the disease do not provide sufficient discriminatory capability, and human infections may be often misdiagnosed. The aim of the current study was to identify the outer membrane Com1 (CBU_1910) as a candidate protein for serodiagnostics of Q fever. The protein was cloned, expressed, purified, and used as an antigen in ELISA. The protein was then used for the screening of sera from patients suffering from chronic Q fever endocarditis, patients whose samples were negative for phase I immunoglobulin G (IgG), patients for whom at least one sample was positive for phase I IgG, and patients suffering from any kind of rheumatoid disease. Blood donors were used as the control group. Following statistical analysis, 92.4% (122/132) of the samples tested agreed with the negative clinical diagnosis, and 72.2% (26/36) agreed with the positive clinical diagnosis. Moreover, a significant correlation to the presence of the disease (p = 0.00) was calculated. The results support the idea that a Com1 antigen-based serodiagnostic test may be useful for differential diagnosis of chronic Q fever. Further studies are required to compare more immunogenic proteins of the bacterium against samples originating from patients suffering from different forms of the disease. MDPI 2019-11-18 /pmc/articles/PMC6963606/ /pubmed/31752191 http://dx.doi.org/10.3390/pathogens8040242 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vranakis, Iosif
Mathioudaki, Eirini
Kokkini, Sofia
Psaroulaki, Anna
Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title_full Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title_fullStr Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title_full_unstemmed Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title_short Com1 as a Promising Protein for the Differential Diagnosis of the Two Forms of Q Fever
title_sort com1 as a promising protein for the differential diagnosis of the two forms of q fever
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963606/
https://www.ncbi.nlm.nih.gov/pubmed/31752191
http://dx.doi.org/10.3390/pathogens8040242
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