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Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography

A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column a...

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Autores principales: Peris-Vicente, Juan, García-Ferrer, Daniel, Mishra, Pooja, Albiol-Chiva, Jaume, Durgbanshi, Abhilasha, Carda-Broch, Samuel, Bose, Devasish, Esteve-Romero, Josep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963619/
https://www.ncbi.nlm.nih.gov/pubmed/31731615
http://dx.doi.org/10.3390/antibiotics8040226
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author Peris-Vicente, Juan
García-Ferrer, Daniel
Mishra, Pooja
Albiol-Chiva, Jaume
Durgbanshi, Abhilasha
Carda-Broch, Samuel
Bose, Devasish
Esteve-Romero, Josep
author_facet Peris-Vicente, Juan
García-Ferrer, Daniel
Mishra, Pooja
Albiol-Chiva, Jaume
Durgbanshi, Abhilasha
Carda-Broch, Samuel
Bose, Devasish
Esteve-Romero, Josep
author_sort Peris-Vicente, Juan
collection PubMed
description A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (R(2) > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control.
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spelling pubmed-69636192020-01-27 Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography Peris-Vicente, Juan García-Ferrer, Daniel Mishra, Pooja Albiol-Chiva, Jaume Durgbanshi, Abhilasha Carda-Broch, Samuel Bose, Devasish Esteve-Romero, Josep Antibiotics (Basel) Article A method based on micellar liquid chromatography was developed to determine oxolinic acid, ciprofloxacin, enrofloxacin, and sarafloxacin in eggs and egg products. The antimicrobial drugs were obtained in a micellar solution which was directly injected. The analytes were resolved using a C18 column and a mobile phase of 0.05 M sodium dodecyl sulfate—7.5% 1-propanol—0.5% triethylamine, buffered at pH 3 with phosphate salt, running under the isocratic mode. The signal was monitored by fluorescence. Validation was successfully performed according to the EU Commission Decision 2002/657/EC in terms of specificity, calibration range (LOQ to 1 mg/kg), linearity (R(2) > 0.9991), limit of detection and decision limit (0.01–0.05 mg/kg), limit of quantification (0.025–0.150 mg/kg), detection capability (<0.4 times decision limit), trueness (−14.2% to +9.8%), precision (<14.0%), robustness, and stability. The procedure was environmentally friendly, safe, easy-to-conduct, inexpensive, and had a high sample throughput, thus it is useful for routine analysis as a screening method in a laboratory for food residue control. MDPI 2019-11-15 /pmc/articles/PMC6963619/ /pubmed/31731615 http://dx.doi.org/10.3390/antibiotics8040226 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peris-Vicente, Juan
García-Ferrer, Daniel
Mishra, Pooja
Albiol-Chiva, Jaume
Durgbanshi, Abhilasha
Carda-Broch, Samuel
Bose, Devasish
Esteve-Romero, Josep
Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title_full Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title_fullStr Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title_full_unstemmed Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title_short Procedure for the Screening of Eggs and Egg Products to Detect Oxolonic Acid, Ciprofloxacin, Enrofloxacin, and Sarafloxacin Using Micellar Liquid Chromatography
title_sort procedure for the screening of eggs and egg products to detect oxolonic acid, ciprofloxacin, enrofloxacin, and sarafloxacin using micellar liquid chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963619/
https://www.ncbi.nlm.nih.gov/pubmed/31731615
http://dx.doi.org/10.3390/antibiotics8040226
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