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Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi

Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the en...

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Autores principales: Calata, Fritz Ivy C., Caranguian, Camille Z., Mendoza, Jillian Ela M., Fornillos, Raffy Jay C., Tabios, Ian Kim B., Fontanilla, Ian Kendrich C., Leonardo, Lydia R., Sunico, Louie S., Kawai, Satoru, Chigusa, Yuichi, Kikuchi, Mihoko, Sato, Megumi, Minamoto, Toshifumi, Baoanan, Zenaida G., Sato, Marcello Otake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963648/
https://www.ncbi.nlm.nih.gov/pubmed/31547610
http://dx.doi.org/10.3390/pathogens8040160
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author Calata, Fritz Ivy C.
Caranguian, Camille Z.
Mendoza, Jillian Ela M.
Fornillos, Raffy Jay C.
Tabios, Ian Kim B.
Fontanilla, Ian Kendrich C.
Leonardo, Lydia R.
Sunico, Louie S.
Kawai, Satoru
Chigusa, Yuichi
Kikuchi, Mihoko
Sato, Megumi
Minamoto, Toshifumi
Baoanan, Zenaida G.
Sato, Marcello Otake
author_facet Calata, Fritz Ivy C.
Caranguian, Camille Z.
Mendoza, Jillian Ela M.
Fornillos, Raffy Jay C.
Tabios, Ian Kim B.
Fontanilla, Ian Kendrich C.
Leonardo, Lydia R.
Sunico, Louie S.
Kawai, Satoru
Chigusa, Yuichi
Kikuchi, Mihoko
Sato, Megumi
Minamoto, Toshifumi
Baoanan, Zenaida G.
Sato, Marcello Otake
author_sort Calata, Fritz Ivy C.
collection PubMed
description Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.
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spelling pubmed-69636482020-01-27 Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi Calata, Fritz Ivy C. Caranguian, Camille Z. Mendoza, Jillian Ela M. Fornillos, Raffy Jay C. Tabios, Ian Kim B. Fontanilla, Ian Kendrich C. Leonardo, Lydia R. Sunico, Louie S. Kawai, Satoru Chigusa, Yuichi Kikuchi, Mihoko Sato, Megumi Minamoto, Toshifumi Baoanan, Zenaida G. Sato, Marcello Otake Pathogens Article Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes. MDPI 2019-09-23 /pmc/articles/PMC6963648/ /pubmed/31547610 http://dx.doi.org/10.3390/pathogens8040160 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Calata, Fritz Ivy C.
Caranguian, Camille Z.
Mendoza, Jillian Ela M.
Fornillos, Raffy Jay C.
Tabios, Ian Kim B.
Fontanilla, Ian Kendrich C.
Leonardo, Lydia R.
Sunico, Louie S.
Kawai, Satoru
Chigusa, Yuichi
Kikuchi, Mihoko
Sato, Megumi
Minamoto, Toshifumi
Baoanan, Zenaida G.
Sato, Marcello Otake
Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title_full Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title_fullStr Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title_full_unstemmed Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title_short Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
title_sort analysis of environmental dna and edaphic factors for the detection of the snail intermediate host oncomelania hupensis quadrasi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963648/
https://www.ncbi.nlm.nih.gov/pubmed/31547610
http://dx.doi.org/10.3390/pathogens8040160
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