Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions

To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount...

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Detalles Bibliográficos
Autores principales: Leite, Gleice, Lawandi, Alexander, Cheng, Matthew P., Lee, Todd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963782/
https://www.ncbi.nlm.nih.gov/pubmed/31557817
http://dx.doi.org/10.3390/antibiotics8040165
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author Leite, Gleice
Lawandi, Alexander
Cheng, Matthew P.
Lee, Todd
author_facet Leite, Gleice
Lawandi, Alexander
Cheng, Matthew P.
Lee, Todd
author_sort Leite, Gleice
collection PubMed
description To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity.
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spelling pubmed-69637822020-01-27 Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions Leite, Gleice Lawandi, Alexander Cheng, Matthew P. Lee, Todd Antibiotics (Basel) Letter To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity. MDPI 2019-09-25 /pmc/articles/PMC6963782/ /pubmed/31557817 http://dx.doi.org/10.3390/antibiotics8040165 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Letter
Leite, Gleice
Lawandi, Alexander
Cheng, Matthew P.
Lee, Todd
Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title_full Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title_fullStr Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title_full_unstemmed Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title_short Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
title_sort stability of biological activity of frozen β-lactams over time as assessed by time-lapsed broth microdilutions
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963782/
https://www.ncbi.nlm.nih.gov/pubmed/31557817
http://dx.doi.org/10.3390/antibiotics8040165
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