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Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions
To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963782/ https://www.ncbi.nlm.nih.gov/pubmed/31557817 http://dx.doi.org/10.3390/antibiotics8040165 |
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author | Leite, Gleice Lawandi, Alexander Cheng, Matthew P. Lee, Todd |
author_facet | Leite, Gleice Lawandi, Alexander Cheng, Matthew P. Lee, Todd |
author_sort | Leite, Gleice |
collection | PubMed |
description | To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity. |
format | Online Article Text |
id | pubmed-6963782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69637822020-01-27 Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions Leite, Gleice Lawandi, Alexander Cheng, Matthew P. Lee, Todd Antibiotics (Basel) Letter To evaluate the antimicrobial agent’s stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity. MDPI 2019-09-25 /pmc/articles/PMC6963782/ /pubmed/31557817 http://dx.doi.org/10.3390/antibiotics8040165 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Letter Leite, Gleice Lawandi, Alexander Cheng, Matthew P. Lee, Todd Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title_full | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title_fullStr | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title_full_unstemmed | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title_short | Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions |
title_sort | stability of biological activity of frozen β-lactams over time as assessed by time-lapsed broth microdilutions |
topic | Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963782/ https://www.ncbi.nlm.nih.gov/pubmed/31557817 http://dx.doi.org/10.3390/antibiotics8040165 |
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