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A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge

Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to...

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Autores principales: Ai, Weicheng, Peng, Zhong, Wang, Fei, Zhang, Yue, Xie, Sisi, Liang, Wan, Hua, Lin, Wang, Xiangru, Chen, Huanchun, Wu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963861/
https://www.ncbi.nlm.nih.gov/pubmed/31690029
http://dx.doi.org/10.3390/vaccines7040176
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author Ai, Weicheng
Peng, Zhong
Wang, Fei
Zhang, Yue
Xie, Sisi
Liang, Wan
Hua, Lin
Wang, Xiangru
Chen, Huanchun
Wu, Bin
author_facet Ai, Weicheng
Peng, Zhong
Wang, Fei
Zhang, Yue
Xie, Sisi
Liang, Wan
Hua, Lin
Wang, Xiangru
Chen, Huanchun
Wu, Bin
author_sort Ai, Weicheng
collection PubMed
description Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on organs. Finally, we demonstrated that vaccination of QH09 activated the systemic as well as the mucosal immunity in pigs and provided protection against lethal bacterial challenge. These findings suggest that the aroA/bscN double deleted B. bronchiseptica QH09 may be an effective vaccine candidate, with safety assurance of animals against B. bronchiseptica infections.
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spelling pubmed-69638612020-01-27 A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge Ai, Weicheng Peng, Zhong Wang, Fei Zhang, Yue Xie, Sisi Liang, Wan Hua, Lin Wang, Xiangru Chen, Huanchun Wu, Bin Vaccines (Basel) Article Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on organs. Finally, we demonstrated that vaccination of QH09 activated the systemic as well as the mucosal immunity in pigs and provided protection against lethal bacterial challenge. These findings suggest that the aroA/bscN double deleted B. bronchiseptica QH09 may be an effective vaccine candidate, with safety assurance of animals against B. bronchiseptica infections. MDPI 2019-11-04 /pmc/articles/PMC6963861/ /pubmed/31690029 http://dx.doi.org/10.3390/vaccines7040176 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ai, Weicheng
Peng, Zhong
Wang, Fei
Zhang, Yue
Xie, Sisi
Liang, Wan
Hua, Lin
Wang, Xiangru
Chen, Huanchun
Wu, Bin
A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title_full A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title_fullStr A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title_full_unstemmed A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title_short A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
title_sort marker-free bordetella bronchiseptica aroa/bscn double deleted mutant confers protection against lethal challenge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963861/
https://www.ncbi.nlm.nih.gov/pubmed/31690029
http://dx.doi.org/10.3390/vaccines7040176
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