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A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge
Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963861/ https://www.ncbi.nlm.nih.gov/pubmed/31690029 http://dx.doi.org/10.3390/vaccines7040176 |
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author | Ai, Weicheng Peng, Zhong Wang, Fei Zhang, Yue Xie, Sisi Liang, Wan Hua, Lin Wang, Xiangru Chen, Huanchun Wu, Bin |
author_facet | Ai, Weicheng Peng, Zhong Wang, Fei Zhang, Yue Xie, Sisi Liang, Wan Hua, Lin Wang, Xiangru Chen, Huanchun Wu, Bin |
author_sort | Ai, Weicheng |
collection | PubMed |
description | Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on organs. Finally, we demonstrated that vaccination of QH09 activated the systemic as well as the mucosal immunity in pigs and provided protection against lethal bacterial challenge. These findings suggest that the aroA/bscN double deleted B. bronchiseptica QH09 may be an effective vaccine candidate, with safety assurance of animals against B. bronchiseptica infections. |
format | Online Article Text |
id | pubmed-6963861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69638612020-01-27 A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge Ai, Weicheng Peng, Zhong Wang, Fei Zhang, Yue Xie, Sisi Liang, Wan Hua, Lin Wang, Xiangru Chen, Huanchun Wu, Bin Vaccines (Basel) Article Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on organs. Finally, we demonstrated that vaccination of QH09 activated the systemic as well as the mucosal immunity in pigs and provided protection against lethal bacterial challenge. These findings suggest that the aroA/bscN double deleted B. bronchiseptica QH09 may be an effective vaccine candidate, with safety assurance of animals against B. bronchiseptica infections. MDPI 2019-11-04 /pmc/articles/PMC6963861/ /pubmed/31690029 http://dx.doi.org/10.3390/vaccines7040176 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ai, Weicheng Peng, Zhong Wang, Fei Zhang, Yue Xie, Sisi Liang, Wan Hua, Lin Wang, Xiangru Chen, Huanchun Wu, Bin A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title | A Marker-Free Bordetella bronchiseptica
aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title_full | A Marker-Free Bordetella bronchiseptica
aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title_fullStr | A Marker-Free Bordetella bronchiseptica
aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title_full_unstemmed | A Marker-Free Bordetella bronchiseptica
aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title_short | A Marker-Free Bordetella bronchiseptica
aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge |
title_sort | marker-free bordetella bronchiseptica
aroa/bscn double deleted mutant confers protection against lethal challenge |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963861/ https://www.ncbi.nlm.nih.gov/pubmed/31690029 http://dx.doi.org/10.3390/vaccines7040176 |
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