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In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been st...

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Autores principales: Cholet, Juliette, Decombat, Caroline, Vareille-Delarbre, Marjolaine, Gainche, Maël, Berry, Alexandre, Senejoux, François, Ripoche, Isabelle, Delort, Laetitia, Vermerie, Marion, Fraisse, Didier, Felgines, Catherine, Ranouille, Edwige, Berthon, Jean-Yves, Priam, Julien, Saunier, Etienne, Tourrette, Albert, Troin, Yves, Thebaud, Gilles, Chalard, Pierre, Caldefie-Chezet, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963863/
https://www.ncbi.nlm.nih.gov/pubmed/31614667
http://dx.doi.org/10.3390/medicines6040101
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author Cholet, Juliette
Decombat, Caroline
Vareille-Delarbre, Marjolaine
Gainche, Maël
Berry, Alexandre
Senejoux, François
Ripoche, Isabelle
Delort, Laetitia
Vermerie, Marion
Fraisse, Didier
Felgines, Catherine
Ranouille, Edwige
Berthon, Jean-Yves
Priam, Julien
Saunier, Etienne
Tourrette, Albert
Troin, Yves
Thebaud, Gilles
Chalard, Pierre
Caldefie-Chezet, Florence
author_facet Cholet, Juliette
Decombat, Caroline
Vareille-Delarbre, Marjolaine
Gainche, Maël
Berry, Alexandre
Senejoux, François
Ripoche, Isabelle
Delort, Laetitia
Vermerie, Marion
Fraisse, Didier
Felgines, Catherine
Ranouille, Edwige
Berthon, Jean-Yves
Priam, Julien
Saunier, Etienne
Tourrette, Albert
Troin, Yves
Thebaud, Gilles
Chalard, Pierre
Caldefie-Chezet, Florence
author_sort Cholet, Juliette
collection PubMed
description Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of Bupleurum rotundifolium roots. Methods: To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of Bupleurum rotundifolium on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs. Results: The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that Bupleurum rotundifolium has immunomodulatory properties. Conclusions: Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation.
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spelling pubmed-69638632020-01-27 In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium Cholet, Juliette Decombat, Caroline Vareille-Delarbre, Marjolaine Gainche, Maël Berry, Alexandre Senejoux, François Ripoche, Isabelle Delort, Laetitia Vermerie, Marion Fraisse, Didier Felgines, Catherine Ranouille, Edwige Berthon, Jean-Yves Priam, Julien Saunier, Etienne Tourrette, Albert Troin, Yves Thebaud, Gilles Chalard, Pierre Caldefie-Chezet, Florence Medicines (Basel) Article Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of Bupleurum rotundifolium roots. Methods: To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of Bupleurum rotundifolium on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs. Results: The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that Bupleurum rotundifolium has immunomodulatory properties. Conclusions: Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation. MDPI 2019-10-11 /pmc/articles/PMC6963863/ /pubmed/31614667 http://dx.doi.org/10.3390/medicines6040101 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cholet, Juliette
Decombat, Caroline
Vareille-Delarbre, Marjolaine
Gainche, Maël
Berry, Alexandre
Senejoux, François
Ripoche, Isabelle
Delort, Laetitia
Vermerie, Marion
Fraisse, Didier
Felgines, Catherine
Ranouille, Edwige
Berthon, Jean-Yves
Priam, Julien
Saunier, Etienne
Tourrette, Albert
Troin, Yves
Thebaud, Gilles
Chalard, Pierre
Caldefie-Chezet, Florence
In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title_full In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title_fullStr In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title_full_unstemmed In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title_short In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium
title_sort in vitro anti-inflammatory and immunomodulatory activities of an extract from the roots of bupleurum rotundifolium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963863/
https://www.ncbi.nlm.nih.gov/pubmed/31614667
http://dx.doi.org/10.3390/medicines6040101
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