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High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae

Klebsiella pneumoniae (Kp), is a frequent cause of hospital and community-acquired infections and WHO had declared it as a “priority pathogen”. Biofilm is a major virulence factor of Kp and yet the mechanism of strong biofilm formation in Kp is unclear. A key objective of the present study is to inv...

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Autores principales: Desai, Siddhi, Sanghrajka, Kinjal, Gajjar, Devarshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963951/
https://www.ncbi.nlm.nih.gov/pubmed/31805671
http://dx.doi.org/10.3390/pathogens8040277
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author Desai, Siddhi
Sanghrajka, Kinjal
Gajjar, Devarshi
author_facet Desai, Siddhi
Sanghrajka, Kinjal
Gajjar, Devarshi
author_sort Desai, Siddhi
collection PubMed
description Klebsiella pneumoniae (Kp), is a frequent cause of hospital and community-acquired infections and WHO had declared it as a “priority pathogen”. Biofilm is a major virulence factor of Kp and yet the mechanism of strong biofilm formation in Kp is unclear. A key objective of the present study is to investigate the differences between strong and weak biofilms formed by clinical isolates of Kp on various catheters and in different media conditions and to identify constituents contributing to strong biofilm formation. Quantification of matrix components (extracellular DNA (eDNA), protein, exopolysaccharides (EPS), and bacterial cells), confocal laser scanning microscopy (CLSM), field emission gun scanning electron microscopy (FEG-SEM) and flow-cytometry analysis were performed to compare strong and weak biofilm matrix. Our results suggest increased biofilm formation on latex catheters compared to silicone and silicone-coated latex catheters. Higher amounts of eDNA, protein, EPS, and dead cells were observed in the strong biofilm of Kp. High adhesion capacity and cell death seem to play a major role in formation of strong Kp biofilms. The enhanced eDNA, EPS, and protein in the biofilm matrix appear as a consequence of increased cell death.
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spelling pubmed-69639512020-01-27 High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae Desai, Siddhi Sanghrajka, Kinjal Gajjar, Devarshi Pathogens Article Klebsiella pneumoniae (Kp), is a frequent cause of hospital and community-acquired infections and WHO had declared it as a “priority pathogen”. Biofilm is a major virulence factor of Kp and yet the mechanism of strong biofilm formation in Kp is unclear. A key objective of the present study is to investigate the differences between strong and weak biofilms formed by clinical isolates of Kp on various catheters and in different media conditions and to identify constituents contributing to strong biofilm formation. Quantification of matrix components (extracellular DNA (eDNA), protein, exopolysaccharides (EPS), and bacterial cells), confocal laser scanning microscopy (CLSM), field emission gun scanning electron microscopy (FEG-SEM) and flow-cytometry analysis were performed to compare strong and weak biofilm matrix. Our results suggest increased biofilm formation on latex catheters compared to silicone and silicone-coated latex catheters. Higher amounts of eDNA, protein, EPS, and dead cells were observed in the strong biofilm of Kp. High adhesion capacity and cell death seem to play a major role in formation of strong Kp biofilms. The enhanced eDNA, EPS, and protein in the biofilm matrix appear as a consequence of increased cell death. MDPI 2019-12-01 /pmc/articles/PMC6963951/ /pubmed/31805671 http://dx.doi.org/10.3390/pathogens8040277 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Desai, Siddhi
Sanghrajka, Kinjal
Gajjar, Devarshi
High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title_full High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title_fullStr High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title_full_unstemmed High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title_short High Adhesion and Increased Cell Death Contribute to Strong Biofilm Formation in Klebsiella pneumoniae
title_sort high adhesion and increased cell death contribute to strong biofilm formation in klebsiella pneumoniae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963951/
https://www.ncbi.nlm.nih.gov/pubmed/31805671
http://dx.doi.org/10.3390/pathogens8040277
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