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Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS
Methicillin-resistant Staphylococcus aureus (MRSA) is a serious pathogen in clinical settings and early detection is critical. Here, we investigated the MRSA discrimination potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using 320 clinical S....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963962/ https://www.ncbi.nlm.nih.gov/pubmed/31683799 http://dx.doi.org/10.3390/pathogens8040214 |
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author | Kim, Jung-Min Kim, Inhee Chung, Sung Hee Chung, Yousun Han, Minje Kim, Jae-Seok |
author_facet | Kim, Jung-Min Kim, Inhee Chung, Sung Hee Chung, Yousun Han, Minje Kim, Jae-Seok |
author_sort | Kim, Jung-Min |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) is a serious pathogen in clinical settings and early detection is critical. Here, we investigated the MRSA discrimination potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using 320 clinical S. aureus isolates obtained in 2005–2014 and 181 isolates obtained in 2018. We conducted polymerase chain reactions (PCR) for staphylococcal cassette chromosome mec (SCCmec) typing and MALDI-TOF MS to find specific markers for methicillin resistance. We identified 21 peaks with significant differences between MRSA and methicillin-susceptible S. aureus (MSSA), as determined by mecA and SCCmec types. Each specific peak was sufficient to discriminate MRSA. We developed two methods for simple discrimination according to these peaks. First, a decision tree for MRSA based on six MRSA-specific peaks, three MSSA-specific peaks, and two SCCmec type IV peaks showed a sensitivity of 96.5%. Second, simple discrimination based on four MRSA-specific peaks and one MSSA peak had a maximum sensitivity of 88.3%. The decision tree applied to 181 S. aureus isolates from 2018 had a sensitivity of 87.6%. In conclusion, we used specific peaks to develop sensitive MRSA identification methods. This rapid and easy MALDI-TOF MS approach can improve patient management. |
format | Online Article Text |
id | pubmed-6963962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69639622020-01-27 Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS Kim, Jung-Min Kim, Inhee Chung, Sung Hee Chung, Yousun Han, Minje Kim, Jae-Seok Pathogens Article Methicillin-resistant Staphylococcus aureus (MRSA) is a serious pathogen in clinical settings and early detection is critical. Here, we investigated the MRSA discrimination potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using 320 clinical S. aureus isolates obtained in 2005–2014 and 181 isolates obtained in 2018. We conducted polymerase chain reactions (PCR) for staphylococcal cassette chromosome mec (SCCmec) typing and MALDI-TOF MS to find specific markers for methicillin resistance. We identified 21 peaks with significant differences between MRSA and methicillin-susceptible S. aureus (MSSA), as determined by mecA and SCCmec types. Each specific peak was sufficient to discriminate MRSA. We developed two methods for simple discrimination according to these peaks. First, a decision tree for MRSA based on six MRSA-specific peaks, three MSSA-specific peaks, and two SCCmec type IV peaks showed a sensitivity of 96.5%. Second, simple discrimination based on four MRSA-specific peaks and one MSSA peak had a maximum sensitivity of 88.3%. The decision tree applied to 181 S. aureus isolates from 2018 had a sensitivity of 87.6%. In conclusion, we used specific peaks to develop sensitive MRSA identification methods. This rapid and easy MALDI-TOF MS approach can improve patient management. MDPI 2019-11-01 /pmc/articles/PMC6963962/ /pubmed/31683799 http://dx.doi.org/10.3390/pathogens8040214 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Jung-Min Kim, Inhee Chung, Sung Hee Chung, Yousun Han, Minje Kim, Jae-Seok Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title | Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title_full | Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title_fullStr | Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title_full_unstemmed | Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title_short | Rapid Discrimination of Methicillin-Resistant Staphylococcus aureus by MALDI-TOF MS |
title_sort | rapid discrimination of methicillin-resistant staphylococcus aureus by maldi-tof ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963962/ https://www.ncbi.nlm.nih.gov/pubmed/31683799 http://dx.doi.org/10.3390/pathogens8040214 |
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