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Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum

[Image: see text] Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases in agriculture. There is no effective control method, although chemical pesticides are used to prevent this disease, but they may lead to serious problems of environmental pollution. N...

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Autores principales: Li, Ping, Yang, Zhongyan, Tang, Bolin, Zhang, Qian, Chen, Zepeng, Zhang, Jili, Wei, Jianyu, Sun, Lirong, Yan, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964269/
https://www.ncbi.nlm.nih.gov/pubmed/31956780
http://dx.doi.org/10.1021/acsomega.9b02746
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author Li, Ping
Yang, Zhongyan
Tang, Bolin
Zhang, Qian
Chen, Zepeng
Zhang, Jili
Wei, Jianyu
Sun, Lirong
Yan, Jian
author_facet Li, Ping
Yang, Zhongyan
Tang, Bolin
Zhang, Qian
Chen, Zepeng
Zhang, Jili
Wei, Jianyu
Sun, Lirong
Yan, Jian
author_sort Li, Ping
collection PubMed
description [Image: see text] Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases in agriculture. There is no effective control method, although chemical pesticides are used to prevent this disease, but they may lead to serious problems of environmental pollution. Natural products from plants can be rich and environmentally friendly sources for a broad spectrum biological control of bacteria. This study focuses on the pericarp of mangosteen (Garcinia mangostana) using bioactivity-guided analysis of different fractions and liquid chromatography–mass spectrometry combined with multivariate analysis to determine markers of active fractions. Six prenyl xanthones, including two new xanthones, garcimangosxanthones H and I, were isolated and identified by NMR and HRESIMS. The biomarker γ-mangostin displayed significant activity against the phytopathogen R. solanacearum with an IC(50) of 34.7 ± 1.5 μg/mL; γ-mangostin affected the bacterial morphology at a concentration of 16.0 μg/mL as seen with a scanning electron microscope image, and it significantly repressed the virulence-associated genes HrpB, FihD, and PilT of R. solanacearum. γ-Mangostin also reduced the symptoms of bacterial wilt disease effectively that is caused by R. solanacearum in tomato and tobacco seedlings in vitro. These results suggested that the use of γ-mangostin from the mangosteen pericarp against R. solanacearum may be used as a natural bacteriostatic agent in agriculture.
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spelling pubmed-69642692020-01-17 Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum Li, Ping Yang, Zhongyan Tang, Bolin Zhang, Qian Chen, Zepeng Zhang, Jili Wei, Jianyu Sun, Lirong Yan, Jian ACS Omega [Image: see text] Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases in agriculture. There is no effective control method, although chemical pesticides are used to prevent this disease, but they may lead to serious problems of environmental pollution. Natural products from plants can be rich and environmentally friendly sources for a broad spectrum biological control of bacteria. This study focuses on the pericarp of mangosteen (Garcinia mangostana) using bioactivity-guided analysis of different fractions and liquid chromatography–mass spectrometry combined with multivariate analysis to determine markers of active fractions. Six prenyl xanthones, including two new xanthones, garcimangosxanthones H and I, were isolated and identified by NMR and HRESIMS. The biomarker γ-mangostin displayed significant activity against the phytopathogen R. solanacearum with an IC(50) of 34.7 ± 1.5 μg/mL; γ-mangostin affected the bacterial morphology at a concentration of 16.0 μg/mL as seen with a scanning electron microscope image, and it significantly repressed the virulence-associated genes HrpB, FihD, and PilT of R. solanacearum. γ-Mangostin also reduced the symptoms of bacterial wilt disease effectively that is caused by R. solanacearum in tomato and tobacco seedlings in vitro. These results suggested that the use of γ-mangostin from the mangosteen pericarp against R. solanacearum may be used as a natural bacteriostatic agent in agriculture. American Chemical Society 2019-12-27 /pmc/articles/PMC6964269/ /pubmed/31956780 http://dx.doi.org/10.1021/acsomega.9b02746 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Li, Ping
Yang, Zhongyan
Tang, Bolin
Zhang, Qian
Chen, Zepeng
Zhang, Jili
Wei, Jianyu
Sun, Lirong
Yan, Jian
Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title_full Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title_fullStr Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title_full_unstemmed Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title_short Identification of Xanthones from the Mangosteen Pericarp that Inhibit the Growth of Ralstonia solanacearum
title_sort identification of xanthones from the mangosteen pericarp that inhibit the growth of ralstonia solanacearum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964269/
https://www.ncbi.nlm.nih.gov/pubmed/31956780
http://dx.doi.org/10.1021/acsomega.9b02746
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