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Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries

[Image: see text] In this study, an inkjet bioprinting-based high-throughput screening (HTS) system was designed and applied for the first time to a catecholpyrimidine-based small molecule library to find hit compounds that inhibit c-Jun NH(2)-terminal kinase1 (JNK1). JNK1 kinase, inactivated MAPKAP...

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Autores principales: Lee, Jungmi, Purushothaman, Baskaran, Song, Joon Myong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964283/
https://www.ncbi.nlm.nih.gov/pubmed/31956806
http://dx.doi.org/10.1021/acsomega.9b03169
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author Lee, Jungmi
Purushothaman, Baskaran
Song, Joon Myong
author_facet Lee, Jungmi
Purushothaman, Baskaran
Song, Joon Myong
author_sort Lee, Jungmi
collection PubMed
description [Image: see text] In this study, an inkjet bioprinting-based high-throughput screening (HTS) system was designed and applied for the first time to a catecholpyrimidine-based small molecule library to find hit compounds that inhibit c-Jun NH(2)-terminal kinase1 (JNK1). JNK1 kinase, inactivated MAPKAPK2, and specific fluorescent peptides along with bioink were printed on parchment paper under optimized printing conditions that did not allow rapid evaporation of printed media based on Triton-X and glycerol. Subsequently, different small compounds were printed and tested against JNK1 kinase to evaluate their degree of phosphorylation inhibition. After printing and incubation, fluorescence intensities from the phosphorylated/nonphosphorylated peptide were acquired for the % phosphorylation analysis. The IM(50) (inhibitory mole 50) value was determined as 1.55 × 10(–15) mol for the hit compound, 22. Thus, this work demonstrated that inkjet bioprinting-based HTS can potentially be adopted for the drug discovery process using small molecule libraries, and cost-effective HTS can be expected to be established based on its low nano- to picoliter printing volume.
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spelling pubmed-69642832020-01-17 Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries Lee, Jungmi Purushothaman, Baskaran Song, Joon Myong ACS Omega [Image: see text] In this study, an inkjet bioprinting-based high-throughput screening (HTS) system was designed and applied for the first time to a catecholpyrimidine-based small molecule library to find hit compounds that inhibit c-Jun NH(2)-terminal kinase1 (JNK1). JNK1 kinase, inactivated MAPKAPK2, and specific fluorescent peptides along with bioink were printed on parchment paper under optimized printing conditions that did not allow rapid evaporation of printed media based on Triton-X and glycerol. Subsequently, different small compounds were printed and tested against JNK1 kinase to evaluate their degree of phosphorylation inhibition. After printing and incubation, fluorescence intensities from the phosphorylated/nonphosphorylated peptide were acquired for the % phosphorylation analysis. The IM(50) (inhibitory mole 50) value was determined as 1.55 × 10(–15) mol for the hit compound, 22. Thus, this work demonstrated that inkjet bioprinting-based HTS can potentially be adopted for the drug discovery process using small molecule libraries, and cost-effective HTS can be expected to be established based on its low nano- to picoliter printing volume. American Chemical Society 2019-12-27 /pmc/articles/PMC6964283/ /pubmed/31956806 http://dx.doi.org/10.1021/acsomega.9b03169 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Lee, Jungmi
Purushothaman, Baskaran
Song, Joon Myong
Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title_full Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title_fullStr Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title_full_unstemmed Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title_short Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries
title_sort inkjet bioprinting on parchment paper for hit identification from small molecule libraries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964283/
https://www.ncbi.nlm.nih.gov/pubmed/31956806
http://dx.doi.org/10.1021/acsomega.9b03169
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