Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was...

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Autores principales: Leal, Élcio, Liang, Ruiying, Liu, Qi, Villanova, Fabiola, Shi, Lijun, Liang, Lin, Li, Jinxiang, Witkin, Steven S., Cui, Shangjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964837/
https://www.ncbi.nlm.nih.gov/pubmed/31945103
http://dx.doi.org/10.1371/journal.pone.0227705
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author Leal, Élcio
Liang, Ruiying
Liu, Qi
Villanova, Fabiola
Shi, Lijun
Liang, Lin
Li, Jinxiang
Witkin, Steven S.
Cui, Shangjin
author_facet Leal, Élcio
Liang, Ruiying
Liu, Qi
Villanova, Fabiola
Shi, Lijun
Liang, Lin
Li, Jinxiang
Witkin, Steven S.
Cui, Shangjin
author_sort Leal, Élcio
collection PubMed
description Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response.
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spelling pubmed-69648372020-01-26 Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis Leal, Élcio Liang, Ruiying Liu, Qi Villanova, Fabiola Shi, Lijun Liang, Lin Li, Jinxiang Witkin, Steven S. Cui, Shangjin PLoS One Research Article Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response. Public Library of Science 2020-01-16 /pmc/articles/PMC6964837/ /pubmed/31945103 http://dx.doi.org/10.1371/journal.pone.0227705 Text en © 2020 Leal et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Leal, Élcio
Liang, Ruiying
Liu, Qi
Villanova, Fabiola
Shi, Lijun
Liang, Lin
Li, Jinxiang
Witkin, Steven S.
Cui, Shangjin
Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title_full Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title_fullStr Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title_full_unstemmed Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title_short Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis
title_sort regional adaptations and parallel mutations in feline panleukopenia virus strains from china revealed by nearly-full length genome analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964837/
https://www.ncbi.nlm.nih.gov/pubmed/31945103
http://dx.doi.org/10.1371/journal.pone.0227705
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