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Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan

Rapeseed (Brassica napus L.) is one of the most economically important oilseed crops worldwide. In Japan, it has been cultivated for more than a century and has formed many feral populations. The aim of this study was to elucidate the genetic diversity of feral rapeseeds by genotyping 537 individual...

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Autores principales: Chen, Ruikun, Shimono, Ayako, Aono, Mitsuko, Nakajima, Nobuyoshi, Ohsawa, Ryo, Yoshioka, Yosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964882/
https://www.ncbi.nlm.nih.gov/pubmed/31945118
http://dx.doi.org/10.1371/journal.pone.0227990
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author Chen, Ruikun
Shimono, Ayako
Aono, Mitsuko
Nakajima, Nobuyoshi
Ohsawa, Ryo
Yoshioka, Yosuke
author_facet Chen, Ruikun
Shimono, Ayako
Aono, Mitsuko
Nakajima, Nobuyoshi
Ohsawa, Ryo
Yoshioka, Yosuke
author_sort Chen, Ruikun
collection PubMed
description Rapeseed (Brassica napus L.) is one of the most economically important oilseed crops worldwide. In Japan, it has been cultivated for more than a century and has formed many feral populations. The aim of this study was to elucidate the genetic diversity of feral rapeseeds by genotyping 537 individuals (among which 130 were determined to be genetically modified) sampled from various regions in Japan. Analysis of 30 microsatellite markers amplified 334 alleles and indicated moderate genetic diversity and high inbreeding (expected heterozygosity, 0.50; observed heterozygosity, 0.16; inbreeding coefficient within individuals, 0.68) within the feral populations. The Mantel test showed only an insignificant weak positive correlation between geographic distance and genetic distance. Analysis of molecular variance showed a greater genetic diversity among individuals than between populations. These results are in accordance with population structure assessed by using principal coordinate analysis and the program STRUCTURE, which showed that the 537 individuals could be assigned to 8 genetic clusters with very large genetic differences among individuals within the same geographic population, and that among feral individuals, many are closely related to rapeseed accessions in the NARO Genebank but some have unknown origins. These unique feral rapeseeds are likely to be affected by strong selection pressure. The results for genetically modified individuals also suggest that they have two different sources and have a considerable degree of diversity, which might be explained by hybridization with nearby individuals and separation of hybrid cultivars. The information obtained in this study could help improve the management of feral rapeseed plants in Japan.
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spelling pubmed-69648822020-01-26 Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan Chen, Ruikun Shimono, Ayako Aono, Mitsuko Nakajima, Nobuyoshi Ohsawa, Ryo Yoshioka, Yosuke PLoS One Research Article Rapeseed (Brassica napus L.) is one of the most economically important oilseed crops worldwide. In Japan, it has been cultivated for more than a century and has formed many feral populations. The aim of this study was to elucidate the genetic diversity of feral rapeseeds by genotyping 537 individuals (among which 130 were determined to be genetically modified) sampled from various regions in Japan. Analysis of 30 microsatellite markers amplified 334 alleles and indicated moderate genetic diversity and high inbreeding (expected heterozygosity, 0.50; observed heterozygosity, 0.16; inbreeding coefficient within individuals, 0.68) within the feral populations. The Mantel test showed only an insignificant weak positive correlation between geographic distance and genetic distance. Analysis of molecular variance showed a greater genetic diversity among individuals than between populations. These results are in accordance with population structure assessed by using principal coordinate analysis and the program STRUCTURE, which showed that the 537 individuals could be assigned to 8 genetic clusters with very large genetic differences among individuals within the same geographic population, and that among feral individuals, many are closely related to rapeseed accessions in the NARO Genebank but some have unknown origins. These unique feral rapeseeds are likely to be affected by strong selection pressure. The results for genetically modified individuals also suggest that they have two different sources and have a considerable degree of diversity, which might be explained by hybridization with nearby individuals and separation of hybrid cultivars. The information obtained in this study could help improve the management of feral rapeseed plants in Japan. Public Library of Science 2020-01-16 /pmc/articles/PMC6964882/ /pubmed/31945118 http://dx.doi.org/10.1371/journal.pone.0227990 Text en © 2020 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Ruikun
Shimono, Ayako
Aono, Mitsuko
Nakajima, Nobuyoshi
Ohsawa, Ryo
Yoshioka, Yosuke
Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title_full Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title_fullStr Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title_full_unstemmed Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title_short Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan
title_sort genetic diversity and population structure of feral rapeseed (brassica napus l.) in japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6964882/
https://www.ncbi.nlm.nih.gov/pubmed/31945118
http://dx.doi.org/10.1371/journal.pone.0227990
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