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An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells

Neurons like other living cells may have ultraweak photon emission (UPE) during neuronal activity. This study is aimed to evaluate UPE from neural stem cells (NSC) during their serial passaging and differentiation. We also investigate whether the addition of silver nanoparticles (AgNPs) or enhanceme...

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Autores principales: Esmaeilpour, Tahereh, Fereydouni, Esmaeil, Dehghani, Farzaneh, Bókkon, Istvan, Panjehshahin, Mohammad-Reza, Császár-Nagy, Noemi, Ranjbar, Mehdi, Salari, Vahid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965084/
https://www.ncbi.nlm.nih.gov/pubmed/31949217
http://dx.doi.org/10.1038/s41598-019-57352-4
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author Esmaeilpour, Tahereh
Fereydouni, Esmaeil
Dehghani, Farzaneh
Bókkon, Istvan
Panjehshahin, Mohammad-Reza
Császár-Nagy, Noemi
Ranjbar, Mehdi
Salari, Vahid
author_facet Esmaeilpour, Tahereh
Fereydouni, Esmaeil
Dehghani, Farzaneh
Bókkon, Istvan
Panjehshahin, Mohammad-Reza
Császár-Nagy, Noemi
Ranjbar, Mehdi
Salari, Vahid
author_sort Esmaeilpour, Tahereh
collection PubMed
description Neurons like other living cells may have ultraweak photon emission (UPE) during neuronal activity. This study is aimed to evaluate UPE from neural stem cells (NSC) during their serial passaging and differentiation. We also investigate whether the addition of silver nanoparticles (AgNPs) or enhancement of UPE (by AgNPs or mirror) affect the differentiation of NSC. In our method, neural stem and progenitor cells of subventricular zone (SVZ) are isolated and expanded using the neurosphere assay. The obtained dissociated cells allocated and cultivated into three groups: groups: I: cell (control), II: cell + mirror, and III: cell + AgNPs. After seven days, the primary neurospheres were counted and their mean number was obtained. Serial passages continuous up to sixth passages in the control group. Differentiation capacity of the resulting neurospheres were evaluated in vitro by immunocytochemistry techniques. Measurement of UPE was carried out by photomultiplier tube (PMT) in the following steps: at the end of primary culture, six serial cell passages of the control group, before and after of the differentiation for 5 minutes. The results show that neither mirror nor AgNPs affect on the neurosphere number. The UPE of the NSC in the sixth subculturing passage was significantly higher than in the primary passage (P < 0.05). AgNPs significantly increased the UPE of the NSC compared to the control group before and after the differentiation (P < 0.05). Also, the treatment with AgNPs increased 44% neuronal differentiation of the harvested NSCs. UPE of NSC after the differentiation was significantly lower than that before the differentiation in each groups, which is in appropriate to the cell numbers (P < 0.0001). The mirror did not significantly increase UPE, neither before nor after the differentiation of NSC. As a conclusion, NSC have UPE-properties and the intensity is increased by serial passaging that are significant in the sixth passage. The AgNPs increases the UPE intensity of NSC that pushes more differentiation of NSC to the neurons. The mirror was not effective in enhancement of UPE. As a result, UPE measurement may be suitable for assessing and studying the effects of nanoparticles in living cells and neurons.
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spelling pubmed-69650842020-01-23 An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells Esmaeilpour, Tahereh Fereydouni, Esmaeil Dehghani, Farzaneh Bókkon, Istvan Panjehshahin, Mohammad-Reza Császár-Nagy, Noemi Ranjbar, Mehdi Salari, Vahid Sci Rep Article Neurons like other living cells may have ultraweak photon emission (UPE) during neuronal activity. This study is aimed to evaluate UPE from neural stem cells (NSC) during their serial passaging and differentiation. We also investigate whether the addition of silver nanoparticles (AgNPs) or enhancement of UPE (by AgNPs or mirror) affect the differentiation of NSC. In our method, neural stem and progenitor cells of subventricular zone (SVZ) are isolated and expanded using the neurosphere assay. The obtained dissociated cells allocated and cultivated into three groups: groups: I: cell (control), II: cell + mirror, and III: cell + AgNPs. After seven days, the primary neurospheres were counted and their mean number was obtained. Serial passages continuous up to sixth passages in the control group. Differentiation capacity of the resulting neurospheres were evaluated in vitro by immunocytochemistry techniques. Measurement of UPE was carried out by photomultiplier tube (PMT) in the following steps: at the end of primary culture, six serial cell passages of the control group, before and after of the differentiation for 5 minutes. The results show that neither mirror nor AgNPs affect on the neurosphere number. The UPE of the NSC in the sixth subculturing passage was significantly higher than in the primary passage (P < 0.05). AgNPs significantly increased the UPE of the NSC compared to the control group before and after the differentiation (P < 0.05). Also, the treatment with AgNPs increased 44% neuronal differentiation of the harvested NSCs. UPE of NSC after the differentiation was significantly lower than that before the differentiation in each groups, which is in appropriate to the cell numbers (P < 0.0001). The mirror did not significantly increase UPE, neither before nor after the differentiation of NSC. As a conclusion, NSC have UPE-properties and the intensity is increased by serial passaging that are significant in the sixth passage. The AgNPs increases the UPE intensity of NSC that pushes more differentiation of NSC to the neurons. The mirror was not effective in enhancement of UPE. As a result, UPE measurement may be suitable for assessing and studying the effects of nanoparticles in living cells and neurons. Nature Publishing Group UK 2020-01-16 /pmc/articles/PMC6965084/ /pubmed/31949217 http://dx.doi.org/10.1038/s41598-019-57352-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Esmaeilpour, Tahereh
Fereydouni, Esmaeil
Dehghani, Farzaneh
Bókkon, Istvan
Panjehshahin, Mohammad-Reza
Császár-Nagy, Noemi
Ranjbar, Mehdi
Salari, Vahid
An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title_full An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title_fullStr An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title_full_unstemmed An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title_short An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
title_sort experimental investigation of ultraweak photon emission from adult murine neural stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965084/
https://www.ncbi.nlm.nih.gov/pubmed/31949217
http://dx.doi.org/10.1038/s41598-019-57352-4
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