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Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

Most measures of lung health independently characterise either global lung function or regional lung structure. The ability to measure airflow and lung function regionally would provide a more specific and physiologically focused means by which to assess and track lung disease in both pre-clinical a...

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Autores principales: Murrie, Rhiannon P., Werdiger, Freda, Donnelley, Martin, Lin, Yu-wei, Carnibella, Richard P., Samarage, Chaminda R., Pinar, Isaac, Preissner, Melissa, Wang, Jiping, Li, Jian, Morgan, Kaye S., Parsons, David W., Dubsky, Stephen, Fouras, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965186/
https://www.ncbi.nlm.nih.gov/pubmed/31949224
http://dx.doi.org/10.1038/s41598-019-57376-w
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author Murrie, Rhiannon P.
Werdiger, Freda
Donnelley, Martin
Lin, Yu-wei
Carnibella, Richard P.
Samarage, Chaminda R.
Pinar, Isaac
Preissner, Melissa
Wang, Jiping
Li, Jian
Morgan, Kaye S.
Parsons, David W.
Dubsky, Stephen
Fouras, Andreas
author_facet Murrie, Rhiannon P.
Werdiger, Freda
Donnelley, Martin
Lin, Yu-wei
Carnibella, Richard P.
Samarage, Chaminda R.
Pinar, Isaac
Preissner, Melissa
Wang, Jiping
Li, Jian
Morgan, Kaye S.
Parsons, David W.
Dubsky, Stephen
Fouras, Andreas
author_sort Murrie, Rhiannon P.
collection PubMed
description Most measures of lung health independently characterise either global lung function or regional lung structure. The ability to measure airflow and lung function regionally would provide a more specific and physiologically focused means by which to assess and track lung disease in both pre-clinical and clinical settings. One approach for achieving regional lung function measurement is via phase contrast X-ray imaging (PCXI), which has been shown to provide highly sensitive, high-resolution images of the lungs and airways in small animals. The detailed images provided by PCXI allow the application of four-dimensional X-ray velocimetry (4DxV) to track lung tissue motion and provide quantitative information on regional lung function. However, until recently synchrotron facilities were required to produce the highly coherent, high-flux X-rays that are required to achieve lung PCXI at a high enough frame rate to capture lung motion. This paper presents the first translation of 4DxV technology from a synchrotron facility into a laboratory setting by using a liquid-metal jet microfocus X-ray source. This source can provide the coherence required for PCXI and enough X-ray flux to image the dynamics of lung tissue motion during the respiratory cycle, which enables production of images compatible with 4DxV analysis. We demonstrate the measurements that can be captured in vivo in live mice using this technique, including regional airflow and tissue expansion. These measurements can inform physiological and biomedical research studies in small animals and assist in the development of new respiratory treatments.
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spelling pubmed-69651862020-01-23 Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source Murrie, Rhiannon P. Werdiger, Freda Donnelley, Martin Lin, Yu-wei Carnibella, Richard P. Samarage, Chaminda R. Pinar, Isaac Preissner, Melissa Wang, Jiping Li, Jian Morgan, Kaye S. Parsons, David W. Dubsky, Stephen Fouras, Andreas Sci Rep Article Most measures of lung health independently characterise either global lung function or regional lung structure. The ability to measure airflow and lung function regionally would provide a more specific and physiologically focused means by which to assess and track lung disease in both pre-clinical and clinical settings. One approach for achieving regional lung function measurement is via phase contrast X-ray imaging (PCXI), which has been shown to provide highly sensitive, high-resolution images of the lungs and airways in small animals. The detailed images provided by PCXI allow the application of four-dimensional X-ray velocimetry (4DxV) to track lung tissue motion and provide quantitative information on regional lung function. However, until recently synchrotron facilities were required to produce the highly coherent, high-flux X-rays that are required to achieve lung PCXI at a high enough frame rate to capture lung motion. This paper presents the first translation of 4DxV technology from a synchrotron facility into a laboratory setting by using a liquid-metal jet microfocus X-ray source. This source can provide the coherence required for PCXI and enough X-ray flux to image the dynamics of lung tissue motion during the respiratory cycle, which enables production of images compatible with 4DxV analysis. We demonstrate the measurements that can be captured in vivo in live mice using this technique, including regional airflow and tissue expansion. These measurements can inform physiological and biomedical research studies in small animals and assist in the development of new respiratory treatments. Nature Publishing Group UK 2020-01-16 /pmc/articles/PMC6965186/ /pubmed/31949224 http://dx.doi.org/10.1038/s41598-019-57376-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Murrie, Rhiannon P.
Werdiger, Freda
Donnelley, Martin
Lin, Yu-wei
Carnibella, Richard P.
Samarage, Chaminda R.
Pinar, Isaac
Preissner, Melissa
Wang, Jiping
Li, Jian
Morgan, Kaye S.
Parsons, David W.
Dubsky, Stephen
Fouras, Andreas
Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title_full Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title_fullStr Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title_full_unstemmed Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title_short Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source
title_sort real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory x-ray source
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965186/
https://www.ncbi.nlm.nih.gov/pubmed/31949224
http://dx.doi.org/10.1038/s41598-019-57376-w
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