Cargando…
“An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process
Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5′-phosphate, 3′-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical a...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965357/ https://www.ncbi.nlm.nih.gov/pubmed/31998749 http://dx.doi.org/10.3389/fmolb.2019.00153 |
_version_ | 1783488625138204672 |
---|---|
author | Serrano-Benítez, Almudena Cortés-Ledesma, Felipe Ruiz, Jose F. |
author_facet | Serrano-Benítez, Almudena Cortés-Ledesma, Felipe Ruiz, Jose F. |
author_sort | Serrano-Benítez, Almudena |
collection | PubMed |
description | Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5′-phosphate, 3′-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical and structural variations and guarantee the formation of ligatable termini. An important distinction is whether the ends are directly “unblocked” by specific enzymatic activities without affecting the integrity of the DNA molecule and its sequence, or whether they are “processed” by unspecific nucleases that remove nucleotides from the termini. DNA end structure and configuration, therefore, shape the repair process, its requirements, and, importantly, its final outcome. Thus, the molecular mechanisms that coordinate and integrate the cellular response to blocked DSBs, although still largely unexplored, can be particularly relevant for maintaining genome integrity and avoiding malignant transformation and cancer. |
format | Online Article Text |
id | pubmed-6965357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69653572020-01-29 “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process Serrano-Benítez, Almudena Cortés-Ledesma, Felipe Ruiz, Jose F. Front Mol Biosci Molecular Biosciences Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5′-phosphate, 3′-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical and structural variations and guarantee the formation of ligatable termini. An important distinction is whether the ends are directly “unblocked” by specific enzymatic activities without affecting the integrity of the DNA molecule and its sequence, or whether they are “processed” by unspecific nucleases that remove nucleotides from the termini. DNA end structure and configuration, therefore, shape the repair process, its requirements, and, importantly, its final outcome. Thus, the molecular mechanisms that coordinate and integrate the cellular response to blocked DSBs, although still largely unexplored, can be particularly relevant for maintaining genome integrity and avoiding malignant transformation and cancer. Frontiers Media S.A. 2020-01-10 /pmc/articles/PMC6965357/ /pubmed/31998749 http://dx.doi.org/10.3389/fmolb.2019.00153 Text en Copyright © 2020 Serrano-Benítez, Cortés-Ledesma and Ruiz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Serrano-Benítez, Almudena Cortés-Ledesma, Felipe Ruiz, Jose F. “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title | “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title_full | “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title_fullStr | “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title_full_unstemmed | “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title_short | “An End to a Means”: How DNA-End Structure Shapes the Double-Strand Break Repair Process |
title_sort | “an end to a means”: how dna-end structure shapes the double-strand break repair process |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965357/ https://www.ncbi.nlm.nih.gov/pubmed/31998749 http://dx.doi.org/10.3389/fmolb.2019.00153 |
work_keys_str_mv | AT serranobenitezalmudena anendtoameanshowdnaendstructureshapesthedoublestrandbreakrepairprocess AT cortesledesmafelipe anendtoameanshowdnaendstructureshapesthedoublestrandbreakrepairprocess AT ruizjosef anendtoameanshowdnaendstructureshapesthedoublestrandbreakrepairprocess |