The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion

Long non-coding RNAs (lncRNAs) are associated with a spectrum of biological processes such as gene regulation on transcriptional and post-transcriptional levels. The HOXA transcript at the distal tip (HOTTIP) lncRNA plays an important role in carcinogenesis; however, the underlying role of HOTTIP in...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Tao, Wang, Helei, Yu, Haiyao, Bi, Miaomiao, Yan, Zhenkun, Hong, Sen, Li, Shiquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965499/
https://www.ncbi.nlm.nih.gov/pubmed/31945724
http://dx.doi.org/10.1016/j.omtn.2019.12.008
_version_ 1783488629830582272
author Liu, Tao
Wang, Helei
Yu, Haiyao
Bi, Miaomiao
Yan, Zhenkun
Hong, Sen
Li, Shiquan
author_facet Liu, Tao
Wang, Helei
Yu, Haiyao
Bi, Miaomiao
Yan, Zhenkun
Hong, Sen
Li, Shiquan
author_sort Liu, Tao
collection PubMed
description Long non-coding RNAs (lncRNAs) are associated with a spectrum of biological processes such as gene regulation on transcriptional and post-transcriptional levels. The HOXA transcript at the distal tip (HOTTIP) lncRNA plays an important role in carcinogenesis; however, the underlying role of HOTTIP in colorectal carcinoma (CRC) remains unknown. The aim of the present study was to evaluate the expression and function of HOTTIP in CRC. In the present study, we analyzed HOTTIP expression levels of CRC patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of HOTTIP by RNA interference was performed to explore its roles in cell proliferation, migration, and invasion. Our results found that HOTTIP was upregulated in human primary CRC tissues. Knockdown of HOTTIP inhibited CRC cell proliferation, migration, and invasion. Above all, knockdown of HOTTIP could represent a rational therapeutic strategy for CRC.
format Online
Article
Text
id pubmed-6965499
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher American Society of Gene & Cell Therapy
record_format MEDLINE/PubMed
spelling pubmed-69654992020-01-22 The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion Liu, Tao Wang, Helei Yu, Haiyao Bi, Miaomiao Yan, Zhenkun Hong, Sen Li, Shiquan Mol Ther Nucleic Acids Article Long non-coding RNAs (lncRNAs) are associated with a spectrum of biological processes such as gene regulation on transcriptional and post-transcriptional levels. The HOXA transcript at the distal tip (HOTTIP) lncRNA plays an important role in carcinogenesis; however, the underlying role of HOTTIP in colorectal carcinoma (CRC) remains unknown. The aim of the present study was to evaluate the expression and function of HOTTIP in CRC. In the present study, we analyzed HOTTIP expression levels of CRC patients in tumor and adjacent normal tissue by real-time quantitative PCR. Knockdown of HOTTIP by RNA interference was performed to explore its roles in cell proliferation, migration, and invasion. Our results found that HOTTIP was upregulated in human primary CRC tissues. Knockdown of HOTTIP inhibited CRC cell proliferation, migration, and invasion. Above all, knockdown of HOTTIP could represent a rational therapeutic strategy for CRC. American Society of Gene & Cell Therapy 2019-12-18 /pmc/articles/PMC6965499/ /pubmed/31945724 http://dx.doi.org/10.1016/j.omtn.2019.12.008 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Liu, Tao
Wang, Helei
Yu, Haiyao
Bi, Miaomiao
Yan, Zhenkun
Hong, Sen
Li, Shiquan
The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title_full The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title_fullStr The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title_full_unstemmed The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title_short The Long Non-coding RNA HOTTIP Is Highly Expressed in Colorectal Cancer and Enhances Cell Proliferation and Invasion
title_sort long non-coding rna hottip is highly expressed in colorectal cancer and enhances cell proliferation and invasion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965499/
https://www.ncbi.nlm.nih.gov/pubmed/31945724
http://dx.doi.org/10.1016/j.omtn.2019.12.008
work_keys_str_mv AT liutao thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT wanghelei thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT yuhaiyao thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT bimiaomiao thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT yanzhenkun thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT hongsen thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT lishiquan thelongnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT liutao longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT wanghelei longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT yuhaiyao longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT bimiaomiao longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT yanzhenkun longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT hongsen longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion
AT lishiquan longnoncodingrnahottipishighlyexpressedincolorectalcancerandenhancescellproliferationandinvasion

Ejemplares similares