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MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2
Glioblastoma is the most common malignant primary brain tumor among adults and one of the most lethal cancers. It is characterized by the deregulation of signaling pathways involving proliferation, growth, survival, and other factors. MicroRNAs (miRNAs) play a role in the regulation of genes by affe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965505/ https://www.ncbi.nlm.nih.gov/pubmed/31954330 http://dx.doi.org/10.1016/j.omtn.2019.11.033 |
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author | Moradimotlagh, Atieh Arefian, Ehsan Rezazadeh Valojerdi, Rezvan Ghaemi, Shokoofeh Jamshidi Adegani, Fatemeh Soleimani, Masoud |
author_facet | Moradimotlagh, Atieh Arefian, Ehsan Rezazadeh Valojerdi, Rezvan Ghaemi, Shokoofeh Jamshidi Adegani, Fatemeh Soleimani, Masoud |
author_sort | Moradimotlagh, Atieh |
collection | PubMed |
description | Glioblastoma is the most common malignant primary brain tumor among adults and one of the most lethal cancers. It is characterized by the deregulation of signaling pathways involving proliferation, growth, survival, and other factors. MicroRNAs (miRNAs) play a role in the regulation of genes by affecting the 3′ untranslated region (UTR) of mRNA and affect many cell functions. The present study showed that miR-129 decreased the expression of retinoblastoma and p53 signaling pathways’ genes, including CDK4, CDK6, and MDM2. The real-time PCR data indicated that expression of CDK4 in U251 and U87 cell lines declined by 69.8% and 47% (p < 0.05), respectively, and expression of CDK6 and MDM2 in U251 cells decreased by 55.3% (p < 0.0001) and 34.7% (p < 0.05), respectively. Luciferase assays confirmed that overexpression of miR-129 decreased the expression of the CDK4 gene by 58.9% (p < 0.01), CDK6 by 35.7% (p < 0.0001), and MDM2 by 49% (p < 0.001). Moreover, cell cycle assays showed a decrease of the G(2)-phase population to 10% and pre-G(2) arrest in U87 cells (p < 0.05). Additionally, wound healing assays indicated that miR-129 overexpression inhibits cell growth of glioblastoma cells. These findings introduced novel targets for miR-129 in glioblastoma cells. |
format | Online Article Text |
id | pubmed-6965505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-69655052020-01-22 MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 Moradimotlagh, Atieh Arefian, Ehsan Rezazadeh Valojerdi, Rezvan Ghaemi, Shokoofeh Jamshidi Adegani, Fatemeh Soleimani, Masoud Mol Ther Nucleic Acids Article Glioblastoma is the most common malignant primary brain tumor among adults and one of the most lethal cancers. It is characterized by the deregulation of signaling pathways involving proliferation, growth, survival, and other factors. MicroRNAs (miRNAs) play a role in the regulation of genes by affecting the 3′ untranslated region (UTR) of mRNA and affect many cell functions. The present study showed that miR-129 decreased the expression of retinoblastoma and p53 signaling pathways’ genes, including CDK4, CDK6, and MDM2. The real-time PCR data indicated that expression of CDK4 in U251 and U87 cell lines declined by 69.8% and 47% (p < 0.05), respectively, and expression of CDK6 and MDM2 in U251 cells decreased by 55.3% (p < 0.0001) and 34.7% (p < 0.05), respectively. Luciferase assays confirmed that overexpression of miR-129 decreased the expression of the CDK4 gene by 58.9% (p < 0.01), CDK6 by 35.7% (p < 0.0001), and MDM2 by 49% (p < 0.001). Moreover, cell cycle assays showed a decrease of the G(2)-phase population to 10% and pre-G(2) arrest in U87 cells (p < 0.05). Additionally, wound healing assays indicated that miR-129 overexpression inhibits cell growth of glioblastoma cells. These findings introduced novel targets for miR-129 in glioblastoma cells. American Society of Gene & Cell Therapy 2019-12-12 /pmc/articles/PMC6965505/ /pubmed/31954330 http://dx.doi.org/10.1016/j.omtn.2019.11.033 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Moradimotlagh, Atieh Arefian, Ehsan Rezazadeh Valojerdi, Rezvan Ghaemi, Shokoofeh Jamshidi Adegani, Fatemeh Soleimani, Masoud MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title | MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title_full | MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title_fullStr | MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title_full_unstemmed | MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title_short | MicroRNA-129 Inhibits Glioma Cell Growth by Targeting CDK4, CDK6, and MDM2 |
title_sort | microrna-129 inhibits glioma cell growth by targeting cdk4, cdk6, and mdm2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965505/ https://www.ncbi.nlm.nih.gov/pubmed/31954330 http://dx.doi.org/10.1016/j.omtn.2019.11.033 |
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