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The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii

Pinctada fucata martensii, is an economically important marine bivalve species cultured for seawater pearls. At present, we know little about the molecular mechanisms of the insulin signalling pathway in this oyster. Herein, we cloned and analysed an insulin-like peptide (PfILP) and its signalling p...

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Autores principales: Zhang, Hua, He, Maoxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965660/
https://www.ncbi.nlm.nih.gov/pubmed/31949275
http://dx.doi.org/10.1038/s41598-019-57329-3
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author Zhang, Hua
He, Maoxian
author_facet Zhang, Hua
He, Maoxian
author_sort Zhang, Hua
collection PubMed
description Pinctada fucata martensii, is an economically important marine bivalve species cultured for seawater pearls. At present, we know little about the molecular mechanisms of the insulin signalling pathway in this oyster. Herein, we cloned and analysed an insulin-like peptide (PfILP) and its signalling pathway-related genes. We detected their expression levels in different tissues and developmental stages. Recombinant PfILP protein was produced and found to significantly increase primary mantle cell activity and induce the expression of the proliferating cell nuclear antigen (PCNA) gene. PfILP could also regulate the 293T cell cycle by stimulating the S phase and inhibiting the G1 and G2 phases. Recombinant PfILP protein induced the expression of its signalling pathway-related genes in mantle cells. In vitro co-immunoprecipitation analysis showed that PfILP interacts with PfIRR. PfILP activated expression of the pfIRR protein, and also activated the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways by stimulating phosphorylation of MAPK and AKT. Further analysis showed that PfILP up-regulated glycogen synthesis-related genes glycogen synthase kinase-3 beta (GSK-3β), protein phosphatase 1 (PP1) and glucokinase (GK) at the mRNA level, as well as the expression of the PP1 protein, and phosphorylation of GSK-3β. These results confirmed the presence of a conserved insulin-like signalling pathway in pearl oyster that is involved in cell activity, glycogen metabolism, and other physiological processes.
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spelling pubmed-69656602020-01-23 The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii Zhang, Hua He, Maoxian Sci Rep Article Pinctada fucata martensii, is an economically important marine bivalve species cultured for seawater pearls. At present, we know little about the molecular mechanisms of the insulin signalling pathway in this oyster. Herein, we cloned and analysed an insulin-like peptide (PfILP) and its signalling pathway-related genes. We detected their expression levels in different tissues and developmental stages. Recombinant PfILP protein was produced and found to significantly increase primary mantle cell activity and induce the expression of the proliferating cell nuclear antigen (PCNA) gene. PfILP could also regulate the 293T cell cycle by stimulating the S phase and inhibiting the G1 and G2 phases. Recombinant PfILP protein induced the expression of its signalling pathway-related genes in mantle cells. In vitro co-immunoprecipitation analysis showed that PfILP interacts with PfIRR. PfILP activated expression of the pfIRR protein, and also activated the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways by stimulating phosphorylation of MAPK and AKT. Further analysis showed that PfILP up-regulated glycogen synthesis-related genes glycogen synthase kinase-3 beta (GSK-3β), protein phosphatase 1 (PP1) and glucokinase (GK) at the mRNA level, as well as the expression of the PP1 protein, and phosphorylation of GSK-3β. These results confirmed the presence of a conserved insulin-like signalling pathway in pearl oyster that is involved in cell activity, glycogen metabolism, and other physiological processes. Nature Publishing Group UK 2020-01-16 /pmc/articles/PMC6965660/ /pubmed/31949275 http://dx.doi.org/10.1038/s41598-019-57329-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Hua
He, Maoxian
The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title_full The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title_fullStr The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title_full_unstemmed The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title_short The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
title_sort role of a new insulin-like peptide in the pearl oyster pinctada fucata martensii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965660/
https://www.ncbi.nlm.nih.gov/pubmed/31949275
http://dx.doi.org/10.1038/s41598-019-57329-3
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