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A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms

Although mammography is the gold standard for breast cancer screening, the high rates of false-positive mammograms remain a concern. Thus, there is an unmet clinical need for a non-invasive and reliable test to differentiate between malignant and benign breast lesions in order to avoid subjecting pa...

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Autores principales: Loke, Sau Yeen, Munusamy, Prabhakaran, Koh, Geok Ling, Chan, Claire Hian Tzer, Madhukumar, Preetha, Thung, Jee Liang, Tan, Kiat Tee Benita, Ong, Kong Wee, Yong, Wei Sean, Sim, Yirong, Oey, Chung Lie, Lim, Sue Zann, Chan, Mun Yew Patrick, Ho, Teng Swan Juliana, Khoo, Boon Kheng James, Wong, Su Lin Jill, Thng, Choon Hua, Chong, Bee Kiang, Tan, Ern Yu, Tan, Veronique Kiak-Mien, Lee, Ann Siew Gek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966622/
https://www.ncbi.nlm.nih.gov/pubmed/31769433
http://dx.doi.org/10.3390/cancers11121872
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author Loke, Sau Yeen
Munusamy, Prabhakaran
Koh, Geok Ling
Chan, Claire Hian Tzer
Madhukumar, Preetha
Thung, Jee Liang
Tan, Kiat Tee Benita
Ong, Kong Wee
Yong, Wei Sean
Sim, Yirong
Oey, Chung Lie
Lim, Sue Zann
Chan, Mun Yew Patrick
Ho, Teng Swan Juliana
Khoo, Boon Kheng James
Wong, Su Lin Jill
Thng, Choon Hua
Chong, Bee Kiang
Tan, Ern Yu
Tan, Veronique Kiak-Mien
Lee, Ann Siew Gek
author_facet Loke, Sau Yeen
Munusamy, Prabhakaran
Koh, Geok Ling
Chan, Claire Hian Tzer
Madhukumar, Preetha
Thung, Jee Liang
Tan, Kiat Tee Benita
Ong, Kong Wee
Yong, Wei Sean
Sim, Yirong
Oey, Chung Lie
Lim, Sue Zann
Chan, Mun Yew Patrick
Ho, Teng Swan Juliana
Khoo, Boon Kheng James
Wong, Su Lin Jill
Thng, Choon Hua
Chong, Bee Kiang
Tan, Ern Yu
Tan, Veronique Kiak-Mien
Lee, Ann Siew Gek
author_sort Loke, Sau Yeen
collection PubMed
description Although mammography is the gold standard for breast cancer screening, the high rates of false-positive mammograms remain a concern. Thus, there is an unmet clinical need for a non-invasive and reliable test to differentiate between malignant and benign breast lesions in order to avoid subjecting patients with abnormal mammograms to unnecessary follow-up diagnostic procedures. Serum samples from 116 malignant breast lesions and 64 benign breast lesions were comprehensively profiled for 2,083 microRNAs (miRNAs) using next-generation sequencing. Of the 180 samples profiled, three outliers were removed based on the principal component analysis (PCA), and the remaining samples were divided into training (n = 125) and test (n = 52) sets at a 70:30 ratio for further analysis. In the training set, significantly differentially expressed miRNAs (adjusted p < 0.01) were identified after correcting for multiple testing using a false discovery rate. Subsequently, a predictive classification model using an eight-miRNA signature and a Bayesian logistic regression algorithm was developed. Based on the receiver operating characteristic (ROC) curve analysis in the test set, the model could achieve an area under the curve (AUC) of 0.9542. Together, this study demonstrates the potential use of circulating miRNAs as an adjunct test to stratify breast lesions in patients with abnormal screening mammograms.
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spelling pubmed-69666222020-02-04 A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms Loke, Sau Yeen Munusamy, Prabhakaran Koh, Geok Ling Chan, Claire Hian Tzer Madhukumar, Preetha Thung, Jee Liang Tan, Kiat Tee Benita Ong, Kong Wee Yong, Wei Sean Sim, Yirong Oey, Chung Lie Lim, Sue Zann Chan, Mun Yew Patrick Ho, Teng Swan Juliana Khoo, Boon Kheng James Wong, Su Lin Jill Thng, Choon Hua Chong, Bee Kiang Tan, Ern Yu Tan, Veronique Kiak-Mien Lee, Ann Siew Gek Cancers (Basel) Article Although mammography is the gold standard for breast cancer screening, the high rates of false-positive mammograms remain a concern. Thus, there is an unmet clinical need for a non-invasive and reliable test to differentiate between malignant and benign breast lesions in order to avoid subjecting patients with abnormal mammograms to unnecessary follow-up diagnostic procedures. Serum samples from 116 malignant breast lesions and 64 benign breast lesions were comprehensively profiled for 2,083 microRNAs (miRNAs) using next-generation sequencing. Of the 180 samples profiled, three outliers were removed based on the principal component analysis (PCA), and the remaining samples were divided into training (n = 125) and test (n = 52) sets at a 70:30 ratio for further analysis. In the training set, significantly differentially expressed miRNAs (adjusted p < 0.01) were identified after correcting for multiple testing using a false discovery rate. Subsequently, a predictive classification model using an eight-miRNA signature and a Bayesian logistic regression algorithm was developed. Based on the receiver operating characteristic (ROC) curve analysis in the test set, the model could achieve an area under the curve (AUC) of 0.9542. Together, this study demonstrates the potential use of circulating miRNAs as an adjunct test to stratify breast lesions in patients with abnormal screening mammograms. MDPI 2019-11-26 /pmc/articles/PMC6966622/ /pubmed/31769433 http://dx.doi.org/10.3390/cancers11121872 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loke, Sau Yeen
Munusamy, Prabhakaran
Koh, Geok Ling
Chan, Claire Hian Tzer
Madhukumar, Preetha
Thung, Jee Liang
Tan, Kiat Tee Benita
Ong, Kong Wee
Yong, Wei Sean
Sim, Yirong
Oey, Chung Lie
Lim, Sue Zann
Chan, Mun Yew Patrick
Ho, Teng Swan Juliana
Khoo, Boon Kheng James
Wong, Su Lin Jill
Thng, Choon Hua
Chong, Bee Kiang
Tan, Ern Yu
Tan, Veronique Kiak-Mien
Lee, Ann Siew Gek
A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title_full A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title_fullStr A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title_full_unstemmed A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title_short A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms
title_sort circulating mirna signature for stratification of breast lesions among women with abnormal screening mammograms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966622/
https://www.ncbi.nlm.nih.gov/pubmed/31769433
http://dx.doi.org/10.3390/cancers11121872
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