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MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9
Metastasis-associated protein 2 (MTA2) was previously known as a requirement to maintain malignant potentials in several human cancers. However, the role of MTA2 in the progression of renal cell carcinoma (RCC) has not yet been delineated. In this study, MTA2 expression was significantly increased i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966675/ https://www.ncbi.nlm.nih.gov/pubmed/31771219 http://dx.doi.org/10.3390/cancers11121851 |
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author | Chen, Yong-Syuan Hung, Tung-Wei Su, Shih-Chi Lin, Chia-Liang Yang, Shun-Fa Lee, Chu-Che Yeh, Chang-Fang Hsieh, Yi-Hsien Tsai, Jen-Pi |
author_facet | Chen, Yong-Syuan Hung, Tung-Wei Su, Shih-Chi Lin, Chia-Liang Yang, Shun-Fa Lee, Chu-Che Yeh, Chang-Fang Hsieh, Yi-Hsien Tsai, Jen-Pi |
author_sort | Chen, Yong-Syuan |
collection | PubMed |
description | Metastasis-associated protein 2 (MTA2) was previously known as a requirement to maintain malignant potentials in several human cancers. However, the role of MTA2 in the progression of renal cell carcinoma (RCC) has not yet been delineated. In this study, MTA2 expression was significantly increased in RCC tissues and cell lines. Increased MTA2 expression was significantly associated with tumour grade (p = 0.002) and was an independent prognostic factor for overall survival with a high RCC tumour grade. MTA2 knockdown inhibited the migration, invasion, and in vivo metastasis of RCC cells without effects on cell proliferation. Regarding molecular mechanisms, MTA2 knockdown reduced the activity, protein level, and mRNA expression of matrix metalloproteinase-9 (MMP-9) in RCC cells. Further analyses demonstrated that patients with lower miR-133b expression had poorer survival rates than those with higher expression from The Cancer Genome Atlas database. Moreover, miR-133b modulated the 3′untranslated region (UTR) of MMP-9 promoter activities and subsequently the migratory and invasive abilities of these dysregulated expressions of MTA2 in RCC cells. The inhibition of MTA2 could contribute to human RCC metastasis by regulating the expression of miR-133b targeting MMP-9 expression. |
format | Online Article Text |
id | pubmed-6966675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69666752020-02-04 MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 Chen, Yong-Syuan Hung, Tung-Wei Su, Shih-Chi Lin, Chia-Liang Yang, Shun-Fa Lee, Chu-Che Yeh, Chang-Fang Hsieh, Yi-Hsien Tsai, Jen-Pi Cancers (Basel) Article Metastasis-associated protein 2 (MTA2) was previously known as a requirement to maintain malignant potentials in several human cancers. However, the role of MTA2 in the progression of renal cell carcinoma (RCC) has not yet been delineated. In this study, MTA2 expression was significantly increased in RCC tissues and cell lines. Increased MTA2 expression was significantly associated with tumour grade (p = 0.002) and was an independent prognostic factor for overall survival with a high RCC tumour grade. MTA2 knockdown inhibited the migration, invasion, and in vivo metastasis of RCC cells without effects on cell proliferation. Regarding molecular mechanisms, MTA2 knockdown reduced the activity, protein level, and mRNA expression of matrix metalloproteinase-9 (MMP-9) in RCC cells. Further analyses demonstrated that patients with lower miR-133b expression had poorer survival rates than those with higher expression from The Cancer Genome Atlas database. Moreover, miR-133b modulated the 3′untranslated region (UTR) of MMP-9 promoter activities and subsequently the migratory and invasive abilities of these dysregulated expressions of MTA2 in RCC cells. The inhibition of MTA2 could contribute to human RCC metastasis by regulating the expression of miR-133b targeting MMP-9 expression. MDPI 2019-11-23 /pmc/articles/PMC6966675/ /pubmed/31771219 http://dx.doi.org/10.3390/cancers11121851 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yong-Syuan Hung, Tung-Wei Su, Shih-Chi Lin, Chia-Liang Yang, Shun-Fa Lee, Chu-Che Yeh, Chang-Fang Hsieh, Yi-Hsien Tsai, Jen-Pi MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title | MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title_full | MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title_fullStr | MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title_full_unstemmed | MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title_short | MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9 |
title_sort | mta2 as a potential biomarker and its involvement in metastatic progression of human renal cancer by mir-133b targeting mmp-9 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966675/ https://www.ncbi.nlm.nih.gov/pubmed/31771219 http://dx.doi.org/10.3390/cancers11121851 |
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