Glycogen Accumulation in Neutrophil Can Be a Marker of Sepsis

BACKGROUND: Sepsis, a systemic inflammatory reaction to infection, is the leading cause of death in the world. The early detection and identification of pro-inflammatory changes in overall metabolism and functioning can help in the proper intervention and control of the inflammatory state, and it will improve the prognosis. AIM: In a resource-limited setting where the biomarkers are not easily accessible, this simple technique is required that can help in the early identification of infection and inflammation. METHODOLOGY: The present study was conducted to find the change in glycogen accumulation and morphological changes during inflammation by preparing a peripheral smear and periodic acid–Schiff (PAS) staining. RESULTS: This study shows that the neutrophil accumulates glycogen granules throughout the cytoplasm with the presence of vacuoles in the cytoplasm, thus increasing the neutrophil size and chromatin dispersion. CONCLUSION: PAS staining can be used as a diagnostic method to detect sepsis.