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Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran

L-asparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. This study was conducted to isolate L-asparaginase-producing endophytic fungi from medicinal plants of family Asteraceae. Seven healthy medicinal plants from family Asteraceae w...

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Autores principales: Hatamzadeh, Sareh, Rahnama, Kamran, Nasrollahnejad, Saeed, Fotouhifar, Khalil Berdi, Hemmati, Khodayar, White, James F., Taliei, Fakhtak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6968492/
https://www.ncbi.nlm.nih.gov/pubmed/31976175
http://dx.doi.org/10.7717/peerj.8309
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author Hatamzadeh, Sareh
Rahnama, Kamran
Nasrollahnejad, Saeed
Fotouhifar, Khalil Berdi
Hemmati, Khodayar
White, James F.
Taliei, Fakhtak
author_facet Hatamzadeh, Sareh
Rahnama, Kamran
Nasrollahnejad, Saeed
Fotouhifar, Khalil Berdi
Hemmati, Khodayar
White, James F.
Taliei, Fakhtak
author_sort Hatamzadeh, Sareh
collection PubMed
description L-asparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. This study was conducted to isolate L-asparaginase-producing endophytic fungi from medicinal plants of family Asteraceae. Seven healthy medicinal plants from family Asteraceae were selected for the isolation of endophytic fungi using standard surface sterilization techniques. A total of 837 isolates belonging to 84 species were comprised of the stem (55.6%), leaf (31.1%), root (10.6%) and flower (2.7%). Initial screening of L-asparaginase-producing endophytes was performed by qualitative plate assay on modified Czapex dox’s agar medium. L-asparaginase activity of fungal endophytes was quantified by the nesslerization method. Identification of endophytic fungi was performed using both morphological characteristics and phylogenetic analyses of DNA sequence data including ribosomal DNA regions of ITS (Internal transcribed spacer) and LSU (partial large subunit rDNA), TEF1 (Translation Elongation Factor) and TUB (β-tubulin). Of the 84 isolates, 38 were able to produce L-asparaginase and their L-asparaginase activities were between 0.019 and 0.492 unit/mL with Fusarium proliferatum being the most potent. L-asparaginase-producing endophytes were identified as species of Plectosphaerella, Fusarium, Stemphylium, Septoria, Alternaria, Didymella, Phoma, Chaetosphaeronema, Sarocladium, Nemania, Epicoccum, Ulocladium and Cladosporium. This study showed that endophytic fungi from Asteraceae members have a high L-asparaginase-producing potential and they can be used as an alternative source for production of anticancer enzymes.
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spelling pubmed-69684922020-01-23 Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran Hatamzadeh, Sareh Rahnama, Kamran Nasrollahnejad, Saeed Fotouhifar, Khalil Berdi Hemmati, Khodayar White, James F. Taliei, Fakhtak PeerJ Mycology L-asparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. This study was conducted to isolate L-asparaginase-producing endophytic fungi from medicinal plants of family Asteraceae. Seven healthy medicinal plants from family Asteraceae were selected for the isolation of endophytic fungi using standard surface sterilization techniques. A total of 837 isolates belonging to 84 species were comprised of the stem (55.6%), leaf (31.1%), root (10.6%) and flower (2.7%). Initial screening of L-asparaginase-producing endophytes was performed by qualitative plate assay on modified Czapex dox’s agar medium. L-asparaginase activity of fungal endophytes was quantified by the nesslerization method. Identification of endophytic fungi was performed using both morphological characteristics and phylogenetic analyses of DNA sequence data including ribosomal DNA regions of ITS (Internal transcribed spacer) and LSU (partial large subunit rDNA), TEF1 (Translation Elongation Factor) and TUB (β-tubulin). Of the 84 isolates, 38 were able to produce L-asparaginase and their L-asparaginase activities were between 0.019 and 0.492 unit/mL with Fusarium proliferatum being the most potent. L-asparaginase-producing endophytes were identified as species of Plectosphaerella, Fusarium, Stemphylium, Septoria, Alternaria, Didymella, Phoma, Chaetosphaeronema, Sarocladium, Nemania, Epicoccum, Ulocladium and Cladosporium. This study showed that endophytic fungi from Asteraceae members have a high L-asparaginase-producing potential and they can be used as an alternative source for production of anticancer enzymes. PeerJ Inc. 2020-01-14 /pmc/articles/PMC6968492/ /pubmed/31976175 http://dx.doi.org/10.7717/peerj.8309 Text en ©2020 Hatamzadeh et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Mycology
Hatamzadeh, Sareh
Rahnama, Kamran
Nasrollahnejad, Saeed
Fotouhifar, Khalil Berdi
Hemmati, Khodayar
White, James F.
Taliei, Fakhtak
Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title_full Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title_fullStr Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title_full_unstemmed Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title_short Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran
title_sort isolation and identification of l-asparaginase-producing endophytic fungi from the asteraceae family plant species of iran
topic Mycology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6968492/
https://www.ncbi.nlm.nih.gov/pubmed/31976175
http://dx.doi.org/10.7717/peerj.8309
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