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Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection

Pathogenicity island excision is a phenomenon that occurs in several Salmonella enterica serovars and other members of the family Enterobacteriaceae. ROD21 is an excisable pathogenicity island found in the chromosome of S. Enteritidis, S. Dublin and S. Typhi among others, which contain several genes...

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Autores principales: Pardo-Roa, Catalina, Salazar, Geraldyne A., Noguera, Loreani P., Salazar-Echegarai, Francisco J., Vallejos, Omar P., Suazo, Isidora D., Schultz, Bárbara M., Coronado-Arrázola, Irenice, Kalergis, Alexis M., Bueno, Susan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6968874/
https://www.ncbi.nlm.nih.gov/pubmed/31800631
http://dx.doi.org/10.1371/journal.ppat.1008152
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author Pardo-Roa, Catalina
Salazar, Geraldyne A.
Noguera, Loreani P.
Salazar-Echegarai, Francisco J.
Vallejos, Omar P.
Suazo, Isidora D.
Schultz, Bárbara M.
Coronado-Arrázola, Irenice
Kalergis, Alexis M.
Bueno, Susan M.
author_facet Pardo-Roa, Catalina
Salazar, Geraldyne A.
Noguera, Loreani P.
Salazar-Echegarai, Francisco J.
Vallejos, Omar P.
Suazo, Isidora D.
Schultz, Bárbara M.
Coronado-Arrázola, Irenice
Kalergis, Alexis M.
Bueno, Susan M.
author_sort Pardo-Roa, Catalina
collection PubMed
description Pathogenicity island excision is a phenomenon that occurs in several Salmonella enterica serovars and other members of the family Enterobacteriaceae. ROD21 is an excisable pathogenicity island found in the chromosome of S. Enteritidis, S. Dublin and S. Typhi among others, which contain several genes encoding virulence-associated proteins. Excision of ROD21 may play a role in the ability of S. Enteritidis to cause a systemic infection in mice. Our previous studies have shown that Salmonella strains unable to excise ROD21 display a reduced ability to colonize the liver and spleen. In this work, we determined the kinetics of ROD21 excision in vivo in C57BL/6 mice and its effect on virulence. We quantified bacterial burden and excision frequency in different portions of the digestive tract and internal organs throughout the infection. We observed that the frequency of ROD21 excision was significantly increased in the bacterial population colonizing mesenteric lymph nodes at early stages of the infective cycle, before 48 hours post-infection. In contrast, excision frequency remained very low in the liver and spleen at these stages. Interestingly, excision increased drastically after 48 h post infection, when intestinal re-infection and mortality begun. Moreover, we observed that the inability to excise ROD21 had a negative effect on S. Enteritidis capacity to translocate from the intestine to deeper organs, which correlates with an abnormal transcription of invA in the S. Enteritidis strain unable to excise ROD21. These results suggest that excision of ROD21 is a genetic mechanism required by S. Enteritidis to produce a successful invasion of the intestinal epithelium, a step required to generate systemic infection in mice.
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spelling pubmed-69688742020-01-26 Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection Pardo-Roa, Catalina Salazar, Geraldyne A. Noguera, Loreani P. Salazar-Echegarai, Francisco J. Vallejos, Omar P. Suazo, Isidora D. Schultz, Bárbara M. Coronado-Arrázola, Irenice Kalergis, Alexis M. Bueno, Susan M. PLoS Pathog Research Article Pathogenicity island excision is a phenomenon that occurs in several Salmonella enterica serovars and other members of the family Enterobacteriaceae. ROD21 is an excisable pathogenicity island found in the chromosome of S. Enteritidis, S. Dublin and S. Typhi among others, which contain several genes encoding virulence-associated proteins. Excision of ROD21 may play a role in the ability of S. Enteritidis to cause a systemic infection in mice. Our previous studies have shown that Salmonella strains unable to excise ROD21 display a reduced ability to colonize the liver and spleen. In this work, we determined the kinetics of ROD21 excision in vivo in C57BL/6 mice and its effect on virulence. We quantified bacterial burden and excision frequency in different portions of the digestive tract and internal organs throughout the infection. We observed that the frequency of ROD21 excision was significantly increased in the bacterial population colonizing mesenteric lymph nodes at early stages of the infective cycle, before 48 hours post-infection. In contrast, excision frequency remained very low in the liver and spleen at these stages. Interestingly, excision increased drastically after 48 h post infection, when intestinal re-infection and mortality begun. Moreover, we observed that the inability to excise ROD21 had a negative effect on S. Enteritidis capacity to translocate from the intestine to deeper organs, which correlates with an abnormal transcription of invA in the S. Enteritidis strain unable to excise ROD21. These results suggest that excision of ROD21 is a genetic mechanism required by S. Enteritidis to produce a successful invasion of the intestinal epithelium, a step required to generate systemic infection in mice. Public Library of Science 2019-12-04 /pmc/articles/PMC6968874/ /pubmed/31800631 http://dx.doi.org/10.1371/journal.ppat.1008152 Text en © 2019 Pardo-Roa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pardo-Roa, Catalina
Salazar, Geraldyne A.
Noguera, Loreani P.
Salazar-Echegarai, Francisco J.
Vallejos, Omar P.
Suazo, Isidora D.
Schultz, Bárbara M.
Coronado-Arrázola, Irenice
Kalergis, Alexis M.
Bueno, Susan M.
Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title_full Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title_fullStr Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title_full_unstemmed Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title_short Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
title_sort pathogenicity island excision during an infection by salmonella enterica serovar enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6968874/
https://www.ncbi.nlm.nih.gov/pubmed/31800631
http://dx.doi.org/10.1371/journal.ppat.1008152
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