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Polypeptide templating for designer hierarchical materials

Despite advances in directing the assembly of biomacromolecules into well-defined nanostructures, leveraging pathway complexity of molecular disorder to order transition while bridging materials fabrication from nano- to macroscale remains a challenge. Here, we present templated crystallization of s...

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Autores principales: Sun, Hui, Marelli, Benedetto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969164/
https://www.ncbi.nlm.nih.gov/pubmed/31953407
http://dx.doi.org/10.1038/s41467-019-14257-0
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author Sun, Hui
Marelli, Benedetto
author_facet Sun, Hui
Marelli, Benedetto
author_sort Sun, Hui
collection PubMed
description Despite advances in directing the assembly of biomacromolecules into well-defined nanostructures, leveraging pathway complexity of molecular disorder to order transition while bridging materials fabrication from nano- to macroscale remains a challenge. Here, we present templated crystallization of structural proteins to nanofabricate hierarchically structured materials up to centimeter scale, using silk fibroin as an example. The process involves the use of ordered peptide supramolecular assemblies as templates to direct the folding and assembly of silk fibroin into nanofibrillar structures. Silk polymorphs can be engineered by varying the peptide seeds used. Modulation of the relative concentration between silk fibroin and peptide seeds, silk fibroin molecular weight and pH allows control over nanofibrils morphologies and mechanical properties. Finally, facile integration of the bottom-up templated crystallization with emerging top-down techniques enables the generation of macroscopic nanostructured materials with potential applications in information storage/encryption, surface functionalization, and printable three-dimensional constructs of customized architecture and controlled anisotropy.
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spelling pubmed-69691642020-01-21 Polypeptide templating for designer hierarchical materials Sun, Hui Marelli, Benedetto Nat Commun Article Despite advances in directing the assembly of biomacromolecules into well-defined nanostructures, leveraging pathway complexity of molecular disorder to order transition while bridging materials fabrication from nano- to macroscale remains a challenge. Here, we present templated crystallization of structural proteins to nanofabricate hierarchically structured materials up to centimeter scale, using silk fibroin as an example. The process involves the use of ordered peptide supramolecular assemblies as templates to direct the folding and assembly of silk fibroin into nanofibrillar structures. Silk polymorphs can be engineered by varying the peptide seeds used. Modulation of the relative concentration between silk fibroin and peptide seeds, silk fibroin molecular weight and pH allows control over nanofibrils morphologies and mechanical properties. Finally, facile integration of the bottom-up templated crystallization with emerging top-down techniques enables the generation of macroscopic nanostructured materials with potential applications in information storage/encryption, surface functionalization, and printable three-dimensional constructs of customized architecture and controlled anisotropy. Nature Publishing Group UK 2020-01-17 /pmc/articles/PMC6969164/ /pubmed/31953407 http://dx.doi.org/10.1038/s41467-019-14257-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sun, Hui
Marelli, Benedetto
Polypeptide templating for designer hierarchical materials
title Polypeptide templating for designer hierarchical materials
title_full Polypeptide templating for designer hierarchical materials
title_fullStr Polypeptide templating for designer hierarchical materials
title_full_unstemmed Polypeptide templating for designer hierarchical materials
title_short Polypeptide templating for designer hierarchical materials
title_sort polypeptide templating for designer hierarchical materials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969164/
https://www.ncbi.nlm.nih.gov/pubmed/31953407
http://dx.doi.org/10.1038/s41467-019-14257-0
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