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The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ

BACKGROUND: With the improvement of bacterial detection, the theory of the sterile female upper reproductive tract has been frequently challenged in recent years. However, thus far, no researchers have used ovaries as study targets. METHODS: Six women who were diagnosed with ovarian cancer were incl...

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Autores principales: Wang, Qi, Zhao, Lanbo, Han, Lu, Fu, Guoxing, Tuo, Xiaoqian, Ma, Sijia, Li, Qing, Wang, Yiran, Liang, Dongxin, Tang, Miaomiao, Sun, Chao, Wang, Qing, Song, Qing, Li, Qiling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969417/
https://www.ncbi.nlm.nih.gov/pubmed/31954395
http://dx.doi.org/10.1186/s13048-019-0603-4
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author Wang, Qi
Zhao, Lanbo
Han, Lu
Fu, Guoxing
Tuo, Xiaoqian
Ma, Sijia
Li, Qing
Wang, Yiran
Liang, Dongxin
Tang, Miaomiao
Sun, Chao
Wang, Qing
Song, Qing
Li, Qiling
author_facet Wang, Qi
Zhao, Lanbo
Han, Lu
Fu, Guoxing
Tuo, Xiaoqian
Ma, Sijia
Li, Qing
Wang, Yiran
Liang, Dongxin
Tang, Miaomiao
Sun, Chao
Wang, Qing
Song, Qing
Li, Qiling
author_sort Wang, Qi
collection PubMed
description BACKGROUND: With the improvement of bacterial detection, the theory of the sterile female upper reproductive tract has been frequently challenged in recent years. However, thus far, no researchers have used ovaries as study targets. METHODS: Six women who were diagnosed with ovarian cancer were included in the cancer group, and ten women who were diagnosed with a noncancerous ovarian condition (including three patients with uterine myoma and seven patients with uterine adenomyosis) were included in the control group. Immunohistochemistry staining using an antibacterial lipopolysaccharide (LPS) antibody was used to confirm the presence of bacteria in the ovarian tissues. In addition, 16S rRNA sequencing was used to compare the differences in the bacteria between ovarian cancer tissues and noncancerous ovarian tissues. BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) were used to predict the functional composition of the bacteria. RESULTS: Bacterial LPS was present in ovarian cancer tissue and noncancerous ovarian tissue, which implied the presence of bacteria in ovarian tissue. When compared to the noncancerous ovarian bacteria at the phylum level, the cancerous ovarian bacteria were composed of increased Aquificae and Planctomycetes and decreased Crenarchaeota. When predicting metagenomes, gene functions associated with the potentially pathogenic and the oxidative stress-tolerant phenotype were enriched in the ovaries of the cancer group. Forty-six significantly different KEGG pathways existed in the ovarian bacteria of the cancer group compared to that of the control group. CONCLUSIONS: Different bacteria compositions were present in cancerous and noncancerous ovarian tissues. TRIAL REGISTRATION: Chines Clinical Trail Registry, CHiCTR1800020018, Registered 11 September 2018, http://www.chictr.org.cn/
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spelling pubmed-69694172020-01-27 The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ Wang, Qi Zhao, Lanbo Han, Lu Fu, Guoxing Tuo, Xiaoqian Ma, Sijia Li, Qing Wang, Yiran Liang, Dongxin Tang, Miaomiao Sun, Chao Wang, Qing Song, Qing Li, Qiling J Ovarian Res Research BACKGROUND: With the improvement of bacterial detection, the theory of the sterile female upper reproductive tract has been frequently challenged in recent years. However, thus far, no researchers have used ovaries as study targets. METHODS: Six women who were diagnosed with ovarian cancer were included in the cancer group, and ten women who were diagnosed with a noncancerous ovarian condition (including three patients with uterine myoma and seven patients with uterine adenomyosis) were included in the control group. Immunohistochemistry staining using an antibacterial lipopolysaccharide (LPS) antibody was used to confirm the presence of bacteria in the ovarian tissues. In addition, 16S rRNA sequencing was used to compare the differences in the bacteria between ovarian cancer tissues and noncancerous ovarian tissues. BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) were used to predict the functional composition of the bacteria. RESULTS: Bacterial LPS was present in ovarian cancer tissue and noncancerous ovarian tissue, which implied the presence of bacteria in ovarian tissue. When compared to the noncancerous ovarian bacteria at the phylum level, the cancerous ovarian bacteria were composed of increased Aquificae and Planctomycetes and decreased Crenarchaeota. When predicting metagenomes, gene functions associated with the potentially pathogenic and the oxidative stress-tolerant phenotype were enriched in the ovaries of the cancer group. Forty-six significantly different KEGG pathways existed in the ovarian bacteria of the cancer group compared to that of the control group. CONCLUSIONS: Different bacteria compositions were present in cancerous and noncancerous ovarian tissues. TRIAL REGISTRATION: Chines Clinical Trail Registry, CHiCTR1800020018, Registered 11 September 2018, http://www.chictr.org.cn/ BioMed Central 2020-01-18 /pmc/articles/PMC6969417/ /pubmed/31954395 http://dx.doi.org/10.1186/s13048-019-0603-4 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Qi
Zhao, Lanbo
Han, Lu
Fu, Guoxing
Tuo, Xiaoqian
Ma, Sijia
Li, Qing
Wang, Yiran
Liang, Dongxin
Tang, Miaomiao
Sun, Chao
Wang, Qing
Song, Qing
Li, Qiling
The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title_full The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title_fullStr The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title_full_unstemmed The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title_short The differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
title_sort differential distribution of bacteria between cancerous and noncancerous ovarian tissues in situ
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969417/
https://www.ncbi.nlm.nih.gov/pubmed/31954395
http://dx.doi.org/10.1186/s13048-019-0603-4
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