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A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process

INTRODUCTION: Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able...

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Autores principales: Tito, Annalisa, Minale, Massimiliano, Riccio, Salvatore, Grieco, Fabrizia, Colucci, Maria Gabriella, Apone, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969697/
https://www.ncbi.nlm.nih.gov/pubmed/32021367
http://dx.doi.org/10.2147/CCID.S216391
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author Tito, Annalisa
Minale, Massimiliano
Riccio, Salvatore
Grieco, Fabrizia
Colucci, Maria Gabriella
Apone, Fabio
author_facet Tito, Annalisa
Minale, Massimiliano
Riccio, Salvatore
Grieco, Fabrizia
Colucci, Maria Gabriella
Apone, Fabio
author_sort Tito, Annalisa
collection PubMed
description INTRODUCTION: Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able to accelerate the spontaneous skin repair mechanisms and without altering the skin normal appearance and functionality. The wound healing process in the skin is finely regulated by several factors and orchestrated mechanisms, which modulate the progression and the fitting of different consequent phases, including haemostasis, inflammation, cell proliferation and tissue remodelling. It was previously shown that a patented Triticum vulgare aqueous extract was able to trigger the skin repair process by stimulating new tissue growth and reducing the expression levels of inflammatory mediators, such as IL-6, TNFα, prostaglandin E2, and nitric oxide. METHODS: Scratch assay was performed in Human Dermal Fibroblasts (HDF). The production of fibronectin was measured by gene expression, protein quantification and localization using specific antibodies in HDF. The polymerization of actin was measured using rhodamin-phalloidin in HDF. The epidermal lipid content was estimated in HaCaT (human spontaneously immortalized keratinocytes) using Nile Red staining and the increasing GBA gene expression and activity was demonstrated by RT-PCR and enzymatic activity assay. RESULTS: In the present study, it was demonstrated that the T. vulgare extract enhanced cell migration inducing the synthesis of fibronectin, new actin polymerization and stimulating the expression of the Hyaluronan Synthase 2. Furthermore it improved the restoration of the epidermal barrier stimulating lipid synthesis. CONCLUSION: In conclusion, we demonstrated that the T. vulgare extract possessed promising potential to be developed as a wound healing promoting agent in skin care and dermatology.
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spelling pubmed-69696972020-02-04 A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process Tito, Annalisa Minale, Massimiliano Riccio, Salvatore Grieco, Fabrizia Colucci, Maria Gabriella Apone, Fabio Clin Cosmet Investig Dermatol Original Research INTRODUCTION: Chronic skin lesions represent a problem of increasing occurrence, mostly due to the global ageing of the world population. Research in skin care and dermatology is constantly looking for new non-invasive solutions, preferably those based on the use of natural certified products, able to accelerate the spontaneous skin repair mechanisms and without altering the skin normal appearance and functionality. The wound healing process in the skin is finely regulated by several factors and orchestrated mechanisms, which modulate the progression and the fitting of different consequent phases, including haemostasis, inflammation, cell proliferation and tissue remodelling. It was previously shown that a patented Triticum vulgare aqueous extract was able to trigger the skin repair process by stimulating new tissue growth and reducing the expression levels of inflammatory mediators, such as IL-6, TNFα, prostaglandin E2, and nitric oxide. METHODS: Scratch assay was performed in Human Dermal Fibroblasts (HDF). The production of fibronectin was measured by gene expression, protein quantification and localization using specific antibodies in HDF. The polymerization of actin was measured using rhodamin-phalloidin in HDF. The epidermal lipid content was estimated in HaCaT (human spontaneously immortalized keratinocytes) using Nile Red staining and the increasing GBA gene expression and activity was demonstrated by RT-PCR and enzymatic activity assay. RESULTS: In the present study, it was demonstrated that the T. vulgare extract enhanced cell migration inducing the synthesis of fibronectin, new actin polymerization and stimulating the expression of the Hyaluronan Synthase 2. Furthermore it improved the restoration of the epidermal barrier stimulating lipid synthesis. CONCLUSION: In conclusion, we demonstrated that the T. vulgare extract possessed promising potential to be developed as a wound healing promoting agent in skin care and dermatology. Dove 2020-01-14 /pmc/articles/PMC6969697/ /pubmed/32021367 http://dx.doi.org/10.2147/CCID.S216391 Text en © 2020 Tito et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Tito, Annalisa
Minale, Massimiliano
Riccio, Salvatore
Grieco, Fabrizia
Colucci, Maria Gabriella
Apone, Fabio
A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title_full A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title_fullStr A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title_full_unstemmed A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title_short A Triticum vulgare Extract Exhibits Regenerating Activity During the Wound Healing Process
title_sort triticum vulgare extract exhibits regenerating activity during the wound healing process
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969697/
https://www.ncbi.nlm.nih.gov/pubmed/32021367
http://dx.doi.org/10.2147/CCID.S216391
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