NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Alcohol dehydrogenases (ADHs) are used in reductive biotransformations for the production of valuable chiral alcohols. In this study, we used a high-throughput screening approach based on the NADPH biosensor pSenSox and fluorescence-activated cell sorting (FACS) to search for variants of the NADPH-d...

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Detalles Bibliográficos
Autores principales: Spielmann, Alina, Brack, Yannik, van Beek, Hugo, Flachbart, Lion, Sundermeyer, Lea, Baumgart, Meike, Bott, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969876/
https://www.ncbi.nlm.nih.gov/pubmed/31955268
http://dx.doi.org/10.1186/s13568-020-0946-7

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