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NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface
Alcohol dehydrogenases (ADHs) are used in reductive biotransformations for the production of valuable chiral alcohols. In this study, we used a high-throughput screening approach based on the NADPH biosensor pSenSox and fluorescence-activated cell sorting (FACS) to search for variants of the NADPH-d...
Autores principales: | Spielmann, Alina, Brack, Yannik, van Beek, Hugo, Flachbart, Lion, Sundermeyer, Lea, Baumgart, Meike, Bott, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969876/ https://www.ncbi.nlm.nih.gov/pubmed/31955268 http://dx.doi.org/10.1186/s13568-020-0946-7 |
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