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Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes
BACKGROUND: The main aims of this cohort study were to measure the intrafamilial risk of transmission, sharing and stability of the most virulent S. mutans genotypes. MATERIAL AND METHODS: A total of 392 clinical isolates of S. mutans obtained from caries-active adults and genotyped to evaluate thei...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medicina Oral S.L.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969966/ https://www.ncbi.nlm.nih.gov/pubmed/31976045 http://dx.doi.org/10.4317/jced.56038 |
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author | Cota, Ana-Lídia-Soares Silva, Valter da Silva, Salete-Moura-Bonifácio |
author_facet | Cota, Ana-Lídia-Soares Silva, Valter da Silva, Salete-Moura-Bonifácio |
author_sort | Cota, Ana-Lídia-Soares |
collection | PubMed |
description | BACKGROUND: The main aims of this cohort study were to measure the intrafamilial risk of transmission, sharing and stability of the most virulent S. mutans genotypes. MATERIAL AND METHODS: A total of 392 clinical isolates of S. mutans obtained from caries-active adults and genotyped to evaluate their transmissibility over time. After extraction of the chromosomal DNA, PCR were performed to detect the genes involved in the production of GbpA (gbpA) and mutacin types I, II, III and IV (mutAI, mutAII, mutAIII and mutAIV). RESULTS: The gbpA, mutAI, mutAII, mutAIII and mutAIV genes were detected in 77.3, 12.5, 51, 16.6 and 89.8% of S. mutans isolates, respectively. The virulence of S. mutans was associated with its transmission (P< 0.01) and stability (P = 0.01), with the most virulent genotypes having higher transmissibility (RR = 1.83, 95% CI 1.44 to 2.32) and higher stability in the oral cavity (RR = 1.52, 95% CI 1.06 to 2.19). CONCLUSIONS: Genotypes with the genetic information to synthesize GbpA and mutacins present an important ecological advantage in the process of colonization by S. mutans; they remain stable among the oral microbiota of the host and favor intrafamilial transmission. Key words:Streptococcus mutans, virulence factors, transmission, dental caries. |
format | Online Article Text |
id | pubmed-6969966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Medicina Oral S.L. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69699662020-01-23 Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes Cota, Ana-Lídia-Soares Silva, Valter da Silva, Salete-Moura-Bonifácio J Clin Exp Dent Research BACKGROUND: The main aims of this cohort study were to measure the intrafamilial risk of transmission, sharing and stability of the most virulent S. mutans genotypes. MATERIAL AND METHODS: A total of 392 clinical isolates of S. mutans obtained from caries-active adults and genotyped to evaluate their transmissibility over time. After extraction of the chromosomal DNA, PCR were performed to detect the genes involved in the production of GbpA (gbpA) and mutacin types I, II, III and IV (mutAI, mutAII, mutAIII and mutAIV). RESULTS: The gbpA, mutAI, mutAII, mutAIII and mutAIV genes were detected in 77.3, 12.5, 51, 16.6 and 89.8% of S. mutans isolates, respectively. The virulence of S. mutans was associated with its transmission (P< 0.01) and stability (P = 0.01), with the most virulent genotypes having higher transmissibility (RR = 1.83, 95% CI 1.44 to 2.32) and higher stability in the oral cavity (RR = 1.52, 95% CI 1.06 to 2.19). CONCLUSIONS: Genotypes with the genetic information to synthesize GbpA and mutacins present an important ecological advantage in the process of colonization by S. mutans; they remain stable among the oral microbiota of the host and favor intrafamilial transmission. Key words:Streptococcus mutans, virulence factors, transmission, dental caries. Medicina Oral S.L. 2020-01-01 /pmc/articles/PMC6969966/ /pubmed/31976045 http://dx.doi.org/10.4317/jced.56038 Text en Copyright: © 2020 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Cota, Ana-Lídia-Soares Silva, Valter da Silva, Salete-Moura-Bonifácio Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title | Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title_full | Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title_fullStr | Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title_full_unstemmed | Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title_short | Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes |
title_sort | virulence of streptococcus mutans: an intrafamilial cohort study on transmission of genotypes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6969966/ https://www.ncbi.nlm.nih.gov/pubmed/31976045 http://dx.doi.org/10.4317/jced.56038 |
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