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In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant
BACKGROUND: Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is im...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970091/ https://www.ncbi.nlm.nih.gov/pubmed/31956941 http://dx.doi.org/10.1186/s43141-019-0017-0 |
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author | Lijalem, Teklebrihan Feyissa, Tileye |
author_facet | Lijalem, Teklebrihan Feyissa, Tileye |
author_sort | Lijalem, Teklebrihan |
collection | PubMed |
description | BACKGROUND: Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. RESULTS: Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. CONCLUSIONS: This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement. |
format | Online Article Text |
id | pubmed-6970091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-69700912020-01-31 In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant Lijalem, Teklebrihan Feyissa, Tileye J Genet Eng Biotechnol Research BACKGROUND: Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. RESULTS: Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. CONCLUSIONS: This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement. Springer Berlin Heidelberg 2020-01-20 /pmc/articles/PMC6970091/ /pubmed/31956941 http://dx.doi.org/10.1186/s43141-019-0017-0 Text en © The Author(s) 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Lijalem, Teklebrihan Feyissa, Tileye In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title | In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_full | In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_fullStr | In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_full_unstemmed | In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_short | In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant |
title_sort | in vitro propagation of securidaca longipedunculata (fresen) from shoot tip: an endangered medicinal plant |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970091/ https://www.ncbi.nlm.nih.gov/pubmed/31956941 http://dx.doi.org/10.1186/s43141-019-0017-0 |
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