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Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages
PURPOSE: Isoborneol has been used in the treatment of cardiovascular disease for several years in China. However, the mechanism is still unclear. The aim of this study was to identify the novel mechanism of isoborneol for its application in atherosclerotic disease. MATERIALS AND METHODS: The whole-g...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970257/ https://www.ncbi.nlm.nih.gov/pubmed/32021101 http://dx.doi.org/10.2147/DDDT.S233013 |
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author | Wang, Yunfei Li, Zhengrong Liu, Boxue Wu, Rumeng Gong, Haifeng Su, Zhanhai Zhang, Shoude |
author_facet | Wang, Yunfei Li, Zhengrong Liu, Boxue Wu, Rumeng Gong, Haifeng Su, Zhanhai Zhang, Shoude |
author_sort | Wang, Yunfei |
collection | PubMed |
description | PURPOSE: Isoborneol has been used in the treatment of cardiovascular disease for several years in China. However, the mechanism is still unclear. The aim of this study was to identify the novel mechanism of isoborneol for its application in atherosclerotic disease. MATERIALS AND METHODS: The whole-genome gene expression profiles of MCF-7 cells treated with/or without isoborneol were detected by mRNA microarray analysis. The degree of similarity between the gene expression profiles was compared with the Connectivity Map (CMAP) database. An MTT assay was used to assess the toxicity of isoborneol on RAW 264.7 cells. Oil red O staining and a Dil-ox-LDL uptake assay in RAW 264.7 cells were also used to detect the accumulation of lipids in the macrophages and the uptake of oxidized low-density lipoprotein (ox-LDL). RESULTS: Isoborneol was proved to have mRNA expression profiles similar to that of ikarugamycin which can inhibit the uptake of ox-LDL. This process has proved to be an important cause of foam cell formation and early atherosclerotic lesions. It is speculated, therefore, that isoborneol may show similar activity to that shown by ikarugamycin. Subsequently, it was shown that RAW 264.7 cells reduced the absorption of ox-LDL and the accumulation of intracellular lipids after treatment with different concentrations of isoborneol. CONCLUSION: The results indicate that isoborneol inhibits macrophage consumption of ox-LDL, thereby preventing the accumulation of lipids in the macrophages. These results provide evidence for the application of isoborneol in atherosclerotic disease. |
format | Online Article Text |
id | pubmed-6970257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69702572020-02-04 Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages Wang, Yunfei Li, Zhengrong Liu, Boxue Wu, Rumeng Gong, Haifeng Su, Zhanhai Zhang, Shoude Drug Des Devel Ther Original Research PURPOSE: Isoborneol has been used in the treatment of cardiovascular disease for several years in China. However, the mechanism is still unclear. The aim of this study was to identify the novel mechanism of isoborneol for its application in atherosclerotic disease. MATERIALS AND METHODS: The whole-genome gene expression profiles of MCF-7 cells treated with/or without isoborneol were detected by mRNA microarray analysis. The degree of similarity between the gene expression profiles was compared with the Connectivity Map (CMAP) database. An MTT assay was used to assess the toxicity of isoborneol on RAW 264.7 cells. Oil red O staining and a Dil-ox-LDL uptake assay in RAW 264.7 cells were also used to detect the accumulation of lipids in the macrophages and the uptake of oxidized low-density lipoprotein (ox-LDL). RESULTS: Isoborneol was proved to have mRNA expression profiles similar to that of ikarugamycin which can inhibit the uptake of ox-LDL. This process has proved to be an important cause of foam cell formation and early atherosclerotic lesions. It is speculated, therefore, that isoborneol may show similar activity to that shown by ikarugamycin. Subsequently, it was shown that RAW 264.7 cells reduced the absorption of ox-LDL and the accumulation of intracellular lipids after treatment with different concentrations of isoborneol. CONCLUSION: The results indicate that isoborneol inhibits macrophage consumption of ox-LDL, thereby preventing the accumulation of lipids in the macrophages. These results provide evidence for the application of isoborneol in atherosclerotic disease. Dove 2020-01-15 /pmc/articles/PMC6970257/ /pubmed/32021101 http://dx.doi.org/10.2147/DDDT.S233013 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Wang, Yunfei Li, Zhengrong Liu, Boxue Wu, Rumeng Gong, Haifeng Su, Zhanhai Zhang, Shoude Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title | Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title_full | Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title_fullStr | Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title_full_unstemmed | Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title_short | Isoborneol Attenuates Low-Density Lipoprotein Accumulation and Foam Cell Formation in Macrophages |
title_sort | isoborneol attenuates low-density lipoprotein accumulation and foam cell formation in macrophages |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970257/ https://www.ncbi.nlm.nih.gov/pubmed/32021101 http://dx.doi.org/10.2147/DDDT.S233013 |
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