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Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress

OBJECTIVES: Allium jesdianum (Aj) is a medicinal plant that has highlighted pharmacological features. In this study, the effects of Aj extract were examined on acetaminophen (APAP)-induced hepatic failure in rats. METHODS: Methanolic fraction of hydro-alcoholic extract of Aj was obtained by silica g...

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Autores principales: Sohrabinezhad, Zohreh, Dastan, Dara, Asl, Sara Soleimani, Nili-Ahmadabadi, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Pharmacopuncture Institute (KPI) 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970575/
https://www.ncbi.nlm.nih.gov/pubmed/31970021
http://dx.doi.org/10.3831/KPI.2019.22.032
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author Sohrabinezhad, Zohreh
Dastan, Dara
Asl, Sara Soleimani
Nili-Ahmadabadi, Amir
author_facet Sohrabinezhad, Zohreh
Dastan, Dara
Asl, Sara Soleimani
Nili-Ahmadabadi, Amir
author_sort Sohrabinezhad, Zohreh
collection PubMed
description OBJECTIVES: Allium jesdianum (Aj) is a medicinal plant that has highlighted pharmacological features. In this study, the effects of Aj extract were examined on acetaminophen (APAP)-induced hepatic failure in rats. METHODS: Methanolic fraction of hydro-alcoholic extract of Aj was obtained by silica gel column chromatography method. Animals were randomly divided into four groups each containing six rats and treated by gavage as follows: the first and second groups received normal saline, the third and fourth groups were received with 50 and 100 mg/kg of Aj extract, respectively. After two consecutive weeks, the groups 2–4 were given a single dose of APAP (2 g/kg). After 48 hours, blood and liver samples were collected for biochemical and histological examinations. RESULTS: The findings of the study demonstrated that APAP caused a significant increase in ALT (P < 0.001), AST (P < 0.001), LDH (P < 0.001), ALP (P < 0.001) serum levels, hepatic lipid peroxidation (LPO; P < 0.001) and nitric oxide (NO; P < 0.001). In this regard, APAP led to the depletion of the total antioxidant capacity (TAC; P < 0.001), glutathione and total thiol groups (TTGs; P < 0.001), and structural change in the liver. In the Aj extract groups, a considerable improvement was found in the hepatic function alongside the histopathologic changes. CONCLUSION: This investigation indicated that the influential effects of Aj extract in APAP-induced hepatic failure might depend on its effect on improving oxidant/antioxidant balance in hepatic tissue.
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spelling pubmed-69705752020-01-22 Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress Sohrabinezhad, Zohreh Dastan, Dara Asl, Sara Soleimani Nili-Ahmadabadi, Amir J Pharmacopuncture Original Article OBJECTIVES: Allium jesdianum (Aj) is a medicinal plant that has highlighted pharmacological features. In this study, the effects of Aj extract were examined on acetaminophen (APAP)-induced hepatic failure in rats. METHODS: Methanolic fraction of hydro-alcoholic extract of Aj was obtained by silica gel column chromatography method. Animals were randomly divided into four groups each containing six rats and treated by gavage as follows: the first and second groups received normal saline, the third and fourth groups were received with 50 and 100 mg/kg of Aj extract, respectively. After two consecutive weeks, the groups 2–4 were given a single dose of APAP (2 g/kg). After 48 hours, blood and liver samples were collected for biochemical and histological examinations. RESULTS: The findings of the study demonstrated that APAP caused a significant increase in ALT (P < 0.001), AST (P < 0.001), LDH (P < 0.001), ALP (P < 0.001) serum levels, hepatic lipid peroxidation (LPO; P < 0.001) and nitric oxide (NO; P < 0.001). In this regard, APAP led to the depletion of the total antioxidant capacity (TAC; P < 0.001), glutathione and total thiol groups (TTGs; P < 0.001), and structural change in the liver. In the Aj extract groups, a considerable improvement was found in the hepatic function alongside the histopathologic changes. CONCLUSION: This investigation indicated that the influential effects of Aj extract in APAP-induced hepatic failure might depend on its effect on improving oxidant/antioxidant balance in hepatic tissue. The Korean Pharmacopuncture Institute (KPI) 2019-12 2019-12-31 /pmc/articles/PMC6970575/ /pubmed/31970021 http://dx.doi.org/10.3831/KPI.2019.22.032 Text en © 2019 Korean Pharmacopuncture Institute This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sohrabinezhad, Zohreh
Dastan, Dara
Asl, Sara Soleimani
Nili-Ahmadabadi, Amir
Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title_full Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title_fullStr Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title_full_unstemmed Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title_short Allium Jesdianum Extract Improve Acetaminophen-Induced Hepatic Failure through Inhibition of Oxidative/Nitrosative Stress
title_sort allium jesdianum extract improve acetaminophen-induced hepatic failure through inhibition of oxidative/nitrosative stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970575/
https://www.ncbi.nlm.nih.gov/pubmed/31970021
http://dx.doi.org/10.3831/KPI.2019.22.032
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