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Improved HaloTag Ligand Enables BRET Imaging With NanoLuc

Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions. The recommended use of HaloTag618 ligand for the NanoLuc-HaloTag BRET pair is versatile for ensemble experiments d...

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Detalles Bibliográficos
Autores principales:	Thirukkumaran, Ovia Margaret, Wang, Congrong, Asouzu, Nnamdi Joseph, Fron, Eduard, Rocha, Susana, Hofkens, Johan, Lavis, Luke D., Mizuno, Hideaki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970966/ https://www.ncbi.nlm.nih.gov/pubmed/31993413 http://dx.doi.org/10.3389/fchem.2019.00938

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