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The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites

The mushroom, Sanghuang is widely used in Asian countries. This medicinal fungus produces diverse bioactive compounds and possesses a potent ability to degrade the wood of the mulberry tree. However, the genes, pathways, and mechanisms that are involved in the biosynthesis of the active compounds an...

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Autores principales: Shao, Ying, Guo, Hongwei, Zhang, Jianping, Liu, Hui, Wang, Kun, Zuo, Song, Xu, Pengfei, Xia, Zhenrong, Zhou, Qiumei, Zhang, Hanghang, Wang, Xiangqing, Chen, Anhui, Wang, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971051/
https://www.ncbi.nlm.nih.gov/pubmed/31993039
http://dx.doi.org/10.3389/fmicb.2019.03035
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author Shao, Ying
Guo, Hongwei
Zhang, Jianping
Liu, Hui
Wang, Kun
Zuo, Song
Xu, Pengfei
Xia, Zhenrong
Zhou, Qiumei
Zhang, Hanghang
Wang, Xiangqing
Chen, Anhui
Wang, Yulong
author_facet Shao, Ying
Guo, Hongwei
Zhang, Jianping
Liu, Hui
Wang, Kun
Zuo, Song
Xu, Pengfei
Xia, Zhenrong
Zhou, Qiumei
Zhang, Hanghang
Wang, Xiangqing
Chen, Anhui
Wang, Yulong
author_sort Shao, Ying
collection PubMed
description The mushroom, Sanghuang is widely used in Asian countries. This medicinal fungus produces diverse bioactive compounds and possesses a potent ability to degrade the wood of the mulberry tree. However, the genes, pathways, and mechanisms that are involved in the biosynthesis of the active compounds and wood degradation by Sanghuang mushroom are still unknown. Here, we report a 34.5 Mb genome—encoding 11,310 predicted genes—of this mushroom. About 16.88% (1909) of the predicted genes have been successfully classified as EuKaryotic Orthologous Groups, and approximately 27.23% (665) of these genes are involved in metabolism. Additionally, a total of 334 genes encoding CAZymes—and their characteristics—were compared with those of the other fungi. Homologous genes involved in triterpenoid, polysaccharide, and flavonoid biosynthesis were identified, and their expression was examined during four developmental stages, 10 and 20 days old mycelia, 1 year old and 3 years old fruiting bodies. Importantly, the lack of chalcone isomerase 1 in the flavonoid biosynthesis pathway suggested that different mechanisms were used in this mushroom to synthesize flavonoids than those used in plants. In addition, 343 transporters and 4 velvet family proteins, involved in regulation, uptake, and redistribution of secondary metabolites, were identified. Genomic analysis of this fungus provides insights into its diverse secondary metabolites, which would be beneficial for the investigation of the medical applications of these pharmacological compounds in the future.
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spelling pubmed-69710512020-01-28 The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites Shao, Ying Guo, Hongwei Zhang, Jianping Liu, Hui Wang, Kun Zuo, Song Xu, Pengfei Xia, Zhenrong Zhou, Qiumei Zhang, Hanghang Wang, Xiangqing Chen, Anhui Wang, Yulong Front Microbiol Microbiology The mushroom, Sanghuang is widely used in Asian countries. This medicinal fungus produces diverse bioactive compounds and possesses a potent ability to degrade the wood of the mulberry tree. However, the genes, pathways, and mechanisms that are involved in the biosynthesis of the active compounds and wood degradation by Sanghuang mushroom are still unknown. Here, we report a 34.5 Mb genome—encoding 11,310 predicted genes—of this mushroom. About 16.88% (1909) of the predicted genes have been successfully classified as EuKaryotic Orthologous Groups, and approximately 27.23% (665) of these genes are involved in metabolism. Additionally, a total of 334 genes encoding CAZymes—and their characteristics—were compared with those of the other fungi. Homologous genes involved in triterpenoid, polysaccharide, and flavonoid biosynthesis were identified, and their expression was examined during four developmental stages, 10 and 20 days old mycelia, 1 year old and 3 years old fruiting bodies. Importantly, the lack of chalcone isomerase 1 in the flavonoid biosynthesis pathway suggested that different mechanisms were used in this mushroom to synthesize flavonoids than those used in plants. In addition, 343 transporters and 4 velvet family proteins, involved in regulation, uptake, and redistribution of secondary metabolites, were identified. Genomic analysis of this fungus provides insights into its diverse secondary metabolites, which would be beneficial for the investigation of the medical applications of these pharmacological compounds in the future. Frontiers Media S.A. 2020-01-14 /pmc/articles/PMC6971051/ /pubmed/31993039 http://dx.doi.org/10.3389/fmicb.2019.03035 Text en Copyright © 2020 Shao, Guo, Zhang, Liu, Wang, Zuo, Xu, Xia, Zhou, Zhang, Wang, Chen and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Shao, Ying
Guo, Hongwei
Zhang, Jianping
Liu, Hui
Wang, Kun
Zuo, Song
Xu, Pengfei
Xia, Zhenrong
Zhou, Qiumei
Zhang, Hanghang
Wang, Xiangqing
Chen, Anhui
Wang, Yulong
The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title_full The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title_fullStr The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title_full_unstemmed The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title_short The Genome of the Medicinal Macrofungus Sanghuang Provides Insights Into the Synthesis of Diverse Secondary Metabolites
title_sort genome of the medicinal macrofungus sanghuang provides insights into the synthesis of diverse secondary metabolites
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971051/
https://www.ncbi.nlm.nih.gov/pubmed/31993039
http://dx.doi.org/10.3389/fmicb.2019.03035
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