Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein

BACKGROUND: Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80–90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only rudimentary. We adapt molecular genetics methods of w...

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Detalles Bibliográficos
Autores principales: Blechert, Oliver, Mei, Huan, Zang, Xiaohui, Zheng, Hailin, Liang, Guanzhao, Liu, Weida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971929/
https://www.ncbi.nlm.nih.gov/pubmed/31959181
http://dx.doi.org/10.1186/s12896-020-0601-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971929/
https://www.ncbi.nlm.nih.gov/pubmed/31959181
http://dx.doi.org/10.1186/s12896-020-0601-z

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