A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis

A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4...

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Autores principales: Kilgour, Samantha L., Kilgour, David P. A., Prasongpholchai, Panward, O'Connor, Peter B., Tosin, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972679/
https://www.ncbi.nlm.nih.gov/pubmed/31596972
http://dx.doi.org/10.1002/chem.201903662
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author Kilgour, Samantha L.
Kilgour, David P. A.
Prasongpholchai, Panward
O'Connor, Peter B.
Tosin, Manuela
author_facet Kilgour, Samantha L.
Kilgour, David P. A.
Prasongpholchai, Panward
O'Connor, Peter B.
Tosin, Manuela
author_sort Kilgour, Samantha L.
collection PubMed
description A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin “minimal” polyketide synthase (PKS), a range of putative ACP‐bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT‐ICR‐MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.
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spelling pubmed-69726792020-01-27 A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis Kilgour, Samantha L. Kilgour, David P. A. Prasongpholchai, Panward O'Connor, Peter B. Tosin, Manuela Chemistry Communications A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba(dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin “minimal” polyketide synthase (PKS), a range of putative ACP‐bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT‐ICR‐MS, providing direct insights on active site accessibility and substrate processing for this enzyme class. John Wiley and Sons Inc. 2019-12-06 2019-12-20 /pmc/articles/PMC6972679/ /pubmed/31596972 http://dx.doi.org/10.1002/chem.201903662 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Kilgour, Samantha L.
Kilgour, David P. A.
Prasongpholchai, Panward
O'Connor, Peter B.
Tosin, Manuela
A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title_full A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title_fullStr A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title_full_unstemmed A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title_short A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
title_sort light‐activated acyl carrier protein “trap” for intermediate capture in type ii iterative polyketide biocatalysis
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972679/
https://www.ncbi.nlm.nih.gov/pubmed/31596972
http://dx.doi.org/10.1002/chem.201903662
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