High-throughput identification of protein functional similarities using a gene-expression-based siRNA screen

A gene expression-based siRNA screen was used to evaluate functional similarity between genetic perturbations to identify functionally similar proteins. A siRNA library (siGenome library, Dharmacon) consisting of multiple siRNAs per gene that have been pooled in to one well per gene was arrayed in a...

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Detalles Bibliográficos
Autores principales: Neilsen, Beth K., Kelly, David L., Chakraborty, Binita, Kim, Hyun Seok, White, Michael A., Lewis, Robert E., Fisher, Kurt W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972743/
https://www.ncbi.nlm.nih.gov/pubmed/31964871
http://dx.doi.org/10.1038/s41597-020-0365-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972743/
https://www.ncbi.nlm.nih.gov/pubmed/31964871
http://dx.doi.org/10.1038/s41597-020-0365-2

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