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Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status
To date, the effects of endurance exercise training on lymphocyte physiology at the kinome level are largely unknown. Therefore, the present study used a highly sensitive peptide-based kinase activity profiling approach to investigate if the basal activity of tyrosine (Tyr) and serine/threonine (Ser...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972788/ https://www.ncbi.nlm.nih.gov/pubmed/31964936 http://dx.doi.org/10.1038/s41598-020-57676-6 |
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author | Alack, Katharina Weiss, Astrid Krüger, Karsten Höret, Mona Schermuly, Ralph Frech, Torsten Eggert, Martin Mooren, Frank-Christoph |
author_facet | Alack, Katharina Weiss, Astrid Krüger, Karsten Höret, Mona Schermuly, Ralph Frech, Torsten Eggert, Martin Mooren, Frank-Christoph |
author_sort | Alack, Katharina |
collection | PubMed |
description | To date, the effects of endurance exercise training on lymphocyte physiology at the kinome level are largely unknown. Therefore, the present study used a highly sensitive peptide-based kinase activity profiling approach to investigate if the basal activity of tyrosine (Tyr) and serine/threonine (Ser/Thr) kinases of human lymphocytes is affected by the aerobic endurance training status. Results revealed that the activity of various tyrosine kinases of the FGFR family and ZAP70 was increased, whereas the activity of multiple Ser/Thr kinases such as IKK(α), CaMK4, PKA(α), PKC(α+δ) (among others) was decreased in lymphocytes of endurance trained athletes (ET). Moreover, functional associations between several differentially regulated kinases in ET-derived lymphocytes were demonstrated by phylogenetic mapping and network analysis. Especially, Ser/Thr kinases of the AGC-kinase (protein kinase A, G, and C) family represent exercise-sensitive key components within the lymphocytes kinase network that may mediate the long-term effects of endurance training. Furthermore, KEGG (Kyoto Encyclopedia of Genes and Genomes) and Reactome pathway analysis indicate that Ras as well as intracellular signaling by second messengers were found to be enriched in the ET individuals. Overall, our data suggest that endurance exercise training improves the adaptive immune competence by modulating the activity of multiple protein kinases in human lymphocytes. |
format | Online Article Text |
id | pubmed-6972788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69727882020-01-27 Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status Alack, Katharina Weiss, Astrid Krüger, Karsten Höret, Mona Schermuly, Ralph Frech, Torsten Eggert, Martin Mooren, Frank-Christoph Sci Rep Article To date, the effects of endurance exercise training on lymphocyte physiology at the kinome level are largely unknown. Therefore, the present study used a highly sensitive peptide-based kinase activity profiling approach to investigate if the basal activity of tyrosine (Tyr) and serine/threonine (Ser/Thr) kinases of human lymphocytes is affected by the aerobic endurance training status. Results revealed that the activity of various tyrosine kinases of the FGFR family and ZAP70 was increased, whereas the activity of multiple Ser/Thr kinases such as IKK(α), CaMK4, PKA(α), PKC(α+δ) (among others) was decreased in lymphocytes of endurance trained athletes (ET). Moreover, functional associations between several differentially regulated kinases in ET-derived lymphocytes were demonstrated by phylogenetic mapping and network analysis. Especially, Ser/Thr kinases of the AGC-kinase (protein kinase A, G, and C) family represent exercise-sensitive key components within the lymphocytes kinase network that may mediate the long-term effects of endurance training. Furthermore, KEGG (Kyoto Encyclopedia of Genes and Genomes) and Reactome pathway analysis indicate that Ras as well as intracellular signaling by second messengers were found to be enriched in the ET individuals. Overall, our data suggest that endurance exercise training improves the adaptive immune competence by modulating the activity of multiple protein kinases in human lymphocytes. Nature Publishing Group UK 2020-01-21 /pmc/articles/PMC6972788/ /pubmed/31964936 http://dx.doi.org/10.1038/s41598-020-57676-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Alack, Katharina Weiss, Astrid Krüger, Karsten Höret, Mona Schermuly, Ralph Frech, Torsten Eggert, Martin Mooren, Frank-Christoph Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title | Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title_full | Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title_fullStr | Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title_full_unstemmed | Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title_short | Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
title_sort | profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972788/ https://www.ncbi.nlm.nih.gov/pubmed/31964936 http://dx.doi.org/10.1038/s41598-020-57676-6 |
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